相关实验视频

Updated: Jun 24, 2025

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus
09:57

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

Published on: December 28, 2016

10.7K

基因沉默在aedine细胞系C6/36和U4.4中使用长双链RNA进行沉默

Bodunrin Omokungbe1,2, Alejandra Centurión1,3, Sabrina Stiehler2

  • 1LOEWE Centre for Translational Biodiversity Genomics (LOEWE TBG), Senckenberganlage 25, 60325, Frankfurt Am Main, Germany.

Parasites & vectors
|June 11, 2024
PubMed
概括

研究人员评估了昆虫细胞中RNA干扰 (RNAi) 的转染试剂. 特定的试剂有效地将长双链RNA (dsRNA) 传递给Aedes albopictus U4.4细胞,使基因沉默,而C6/36细胞显示RNAi反应受损.

关键词:
艾迪斯白斑虫 (Aedes albopictus) 是一个白斑虫.艾伯病毒 (Arbovirus) 是一种病毒.细胞培养培养的细胞培养.它们是RNAiRNAi.塞米利基森林病毒转化试剂 转化试剂矢量 矢量 是一个向量.dsRNARNA 是一个mCherry 桃是一种很好的水果.

更多相关视频

Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli08:25

Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli

Published on: March 20, 2016

12.6K
Preparation of Cell-lines for Conditional Knockdown of Gene Expression and Measurement of the Knockdown Effects on E4orf4-Induced Cell Death13:54

Preparation of Cell-lines for Conditional Knockdown of Gene Expression and Measurement of the Knockdown Effects on E4orf4-Induced Cell Death

Published on: October 21, 2012

11.0K

相关概念视频

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.1K
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.0K
RNA Editing02:23

RNA Editing

RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.0K
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
16.7K