一种KNOX II转录因子抑制了大米中NLR免疫受体的BRG8介导免疫力
Siliang Xu1, Xinghua Wei2, Qinqin Yang1
1China National Center for Rice Improvement/State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.
Plant communications
|June 12, 2024
概括
一个新的大米基因,爆发抵抗基因8 (BRG8),增强了植物对爆发和爆发的免疫力. 它由HOS59蛋白调节的调节微调了防御反应,为植物免疫机制提供了新的见解.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物病理学 植物病理学
- 遗传学 遗传学 是一个
背景情况:
- 核酸结合部位和富含白的重复蛋白 (NLR) 是植物免疫的关键调节者,检测病原体效应物以触发防御反应.
- 根据NLR触发免疫的精确机制以及特定NLR在疾病耐药性中的作用尚未完全阐明.
- 了解这些途径对于开发具有对破坏性农业病原体增强抵抗力的作物至关重要.
研究的目的:
- 识别和表征涉及植物防御大米爆发和细菌瘟疫的新型NLR基因.
- 阐明已识别的NLR基因 - - 爆发抗性基因8 (BRG8) 的功能和调控机制.
- 研究BRG8与其他蛋白质的相互作用及其在调节植物免疫反应中的作用.
主要方法:
- 全基因组关联研究 (GWAS) 以确定候选耐药基因.
- 基因过度表达和补充测试以验证功能.
- 亚细胞局部化研究和蛋白质与蛋白质相互作用分析 (共免疫沉).
- 在特定的遗传背景中分析淘汰和过度表达突变.
- 研究蛋白质降解途径 (26S蛋白质组).
主要成果:
- 一个新的NLR基因,爆发抗性基因8 (BRG8) 被确定,赋予了对大米爆发和细菌爆发的抗性.
- BRG8在细胞质和核中都局部化,核局部化增强了米的免疫力,没有过敏反应 (HR).
- BRG8与KNOX II蛋白HOS59相互作用,该蛋白通过蛋白质酶体降解来负面调节BRG8的稳定性和功能.
- HOS59的淘汰会增强BRG8介导的抗性,而HOS59的过度表达会损害它.
结论:
- BRG8是一种新型NLR蛋白,在大米对主要病原体的免疫力中起着重要作用.
- HOS59充当负调节器和免疫检查点,通过有针对性的降解微调BRG8-介导的防御反应.
- 这项研究为NLR基因功能和调节提供了新的见解,突出了植物免疫的复杂机制和作物改善的潜在目标.
相关概念视频
Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K
NF-κB-dependent Signaling Pathway
7.4K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
NF-κB-dependent Signaling Mechanism
The...
7.4K
Co-activators and Co-repressors
7.3K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
7.3K
lncRNA - Long Non-coding RNAs
8.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K
Types of RNA
63.5K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
63.5K
Regulation of the Unfolded Protein Response
2.4K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.4K


