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结合CRISPR-Cas12f的创新传递系统,用于对抗抗菌素耐药性在阴性细菌

Teng-Fei Long^1,2, Shi-Ying Zhou^1,2, Zi-Lei Huang^1,2

¹Guangdong Laboratory for Lingnan Modern Agriculture, National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, P. R. China.

ACS synthetic biology

|June 12, 2024

概括

研究人员开发了pQ-mini,这是一个新的等离子体系统,用于传递CRISPR-Cas12f以对抗抗菌素耐药性. 该系统有效地治疗各种细菌中的抗性基因,为应对这一全球健康威胁提供了一个有希望的新工具.

科学领域:

分子生物学分子生物学
微生物学微生物学
基因工程是一种基因工程.

背景情况:

抗菌素耐药性 (AMR) 是一个关键的全球卫生问题.
克里斯普尔-卡斯系统为基因治愈提供了潜在的潜力,以对抗AMR.
为了广泛应用CRISPR-CasAMR策略,需要有效的传递系统.

研究的目的:

为CRISPR-Cas系统设计和制造一种新的,宽主机范围的基因传递等离子体.
为了使各种细菌中的抗微生物药物耐药性基因能够有效地治愈基因.
评估新系统对临床相关的耐药基因的疗效.

主要方法:

使用Inc.Q等离子体骨干构建一个新的等离子体,pQ-mini.
将CRISPR-Cas12f系统集成到pQ-迷你等离子体中.
评估基因转移效率和各种细菌物种的结合,包括肠杆菌.
对等离子体传播抗微生物耐药性基因 (mcr-1,blaKPC) 治愈效率的评估.

主要成果:

该pQ-迷你等离子体证明了在广泛的细菌宿主范围内有效地转移遗传元素.
pQ-mini的结合效率超过了常见的类似pMB1的等离子体,特别是在肠杆菌中.
pQ-mini成功地将CRISPR-Cas12f系统传递给耐药菌株.
对mcr-1和blaKPC基因实现了高治愈效率,与现有系统相比.

结论:

pQ-mini是一种有效的宽主机范围功能基因传递载体.
该pQ-mini/CRISPR-Cas12f系统显示了广泛的宿主提供抗菌战略的巨大潜力.
这项技术代表了一种有前途的新工具,用于打击抗微生物药物耐药性不断升级的威胁.

关键词:

在 ARG 中,有 ARG 的存在.这就是CRISPR-Cas.这是一种类似Inc.Q的等离子体.宽主机范围的交付系统.

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