开放场测定受室温和影响核心体温的药物的影响
Jessica A Jimenez1, Eric S McCoy2,3, David F Lee2
1UNC Curriculum in Toxicology and Environmental Medicine, The University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, USA.
F1000Research
|June 12, 2024
概括
环境温度在开放场测试中显著影响动物的行为. 核心体温 (CBT) 和环境温度的变化会影响运动活动和与焦虑相关的测量,从而影响研究结果.
科学领域:
- 神经科学是一个神经科学.
- 行为药理学行为药理学
背景情况:
- 开放场测试是评估动物焦虑和药物效应的标准方法.
- 实验室中的环境温度可能会波动,这可能会给行为研究带来变化.
研究的目的:
- 为了研究不同环境和核心体温 (CBT) 对野生类型 (WT) 和短暂受体潜在子家族M成员8 (Trpm8) 敲除小鼠的开场行为的影响.
- 评估温度敏感TRPM8通道对行为反应的影响.
主要方法:
- 测试小鼠 (WT和Trpm8淘汰赛) 在一个开放的场地,在4°C和23°C的温度下进行了1小时的测试.
- 测量了机动活动 (行驶距离) 和中心时间.
- 小鼠接受了易西林,M8-B (TRPM8抗剂),迪亚泽帕姆或盐水的注射.
主要成果:
- 冷却剂 (乙素,4°C环境温度) 降低了WT小鼠的活性和中心时间,效果由TRPM8调节.
- 在WT小鼠中,TRPM8抗剂 (M8-B) 降低了CBT,并在4°C时增加了活性.
- 石降低了CBT,并根据温度不同地改变了WT和Trpm8淘汰小鼠的行为.
结论:
- 环境温度和影响CBT的药物是影响开放场测定结果的关键因素.
- 环境和核心体温都代表了动物行为研究中可变性的重要来源.
- 了解温度的作用对于准确解释开放场测定数据至关重要.
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