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作为一种工具,NIR发射碳纳米颗粒可以使用超分辨率显微镜标记核糖体RNA和核细胞组件

Rohan Lamba¹, Abdul Salam¹, Farhan Anjum²

¹School of Chemical Sciences, Indian Institute of Technology Mandi, H.P.-175075, India. chayan@iitmandi.ac.in.

|June 12, 2024

概括

研究人员开发了新的碳纳米点 (CND),用于在活细胞和固定细胞中以光标记核糖体RNA (rRNA). 这些CND能够对rRNA进行高级成像,有助于理解细胞过程和疾病.

科学领域:

生物技术是生物技术.
细胞生物学细胞生物学
纳米材料是一种纳米材料.

背景情况:

核糖体RNA (rRNA) 对于蛋白质合成和核糖体生物生成至关重要.
目前的rRNA染色方法,如SYTO RNASelect,仅限于固定细胞.
需要先进的光生物标志物用于活细胞rRNA成像.

研究的目的:

合成近红外 (NIR) 发射的新型碳纳米点 (CND) 作为rRNA的光生物标志物.
评估CND在活细胞和固定真核细胞中染色rRNA的有效性.
使用超分辨率显微镜 (SRM) 可视化rRNA分布和动态.

主要方法:

合成NIR发射,光稳定和生物相容的碳纳米点 (CND).
使用合成的CNDs在活细胞和固定真核细胞中染色rRNA.
超分辨率显微镜 (SRM) 用于对rRNA定位的高分辨率成像.
在核细胞,核膜和粗的内等质网膜 (RER) 中分析rRNA分布.

主要成果:

在活细胞和固定细胞中,CNDs成功染色了rRNA.
SRM揭示了核体,核膜和RER.密集纤维元件 (DFCs) 中详细的rRNA定位.
高分辨率成像显示了特定结构,如空洞环结构的DFCs (FWHM 140 nm),核膜 (FWHM 120 nm) 和ER (FWHM 115 nm).
在癌症和正常细胞之间观察到前RNA合成的显著差异.

结论:

开发的CND是活细胞和固定细胞rRNA成像的有效光生物标志物.
通过使用SRM,CNDs可促进使用SRM在各种细胞区中的rRNA的高分辨率可视化.
这些CND具有研究生物过程,疾病发展和药物相互作用中的rRNA动态的潜力.

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