混合PNA-酸水凝作为可注射的CEST-MRI剂
Elisabetta Rosa1, Enza Di Gregorio2, Giuseppe Ferrauto2
1Department of Pharmacy and Interuniversity Research Centre on Bioactive Peptides (CIRPeB) "Carlo Pedone", University of Naples "Federico II", Via D. Montesano 49, Naples 80131, Italy. antonella.accardo@unina.it.
Journal of materials chemistry. B
|June 12, 2024
概括
研究人员开发了新的基于的水凝,用于生物医学成像. 这些材料产生化学交换和转移 (CEST) 的MRI信号,并显示出用于体内癌症检测的可注射植入物的潜力.
科学领域:
- 生物材料科学 生物材料科学
- 化学生物学 化学生物学
- 医疗成像医学成像
背景情况:
- 体自组装为生物医学应用提供了多功能平台.
- 化学交换和转移 (CEST) 磁共振成像 (MRI) 是一种有前途的无金属对比成像技术.
- 开发用于增强MRI对比度和有针对性的交付的新材料至关重要.
研究的目的:
- 为生物医学应用合成和表征核酸 (PNA) 修饰的.
- 为了研究这些基于的水凝的凝性质和机械反应性.
- 评估这些水凝作为CEST-MRI的对比剂以及用于体内癌症检测的可注射植入物的潜力.
主要方法:
- 合成和PNA修饰的含酸的特征 (a-K2,c-K2,g-K2,t-K2).
- 评估水凝形成和机械性能,包括沃森-克里克配对效应.
- 在GL261,TS/a和3T3-NIH细胞系上进行CEST-MRI信号生成分析和体外细胞兼容性测试.
- 在TS/a乳腺癌异种移植的小鼠中通过内注射进行体内评估.
主要成果:
- 该c-K2序列表现出凝性质.
- 沃森-克里克c-K2和g-K2之间的配对显著提高了水凝的机械响应.
- 水凝矩阵从水中产生了可检测的CEST信号,大约为2.5 ppm.
- 这些材料与经过测试的癌症和纤维细胞细胞系表现出细胞相容性.
- 在小鼠乳腺癌模型中,通过内注射获得了成功的体内检测.
结论:
- 经过PNA修饰的基凝可以设计为可调节的机械性能和CEST-MRI信号生成.
- 这些基于的矩阵是细胞兼容的,适合体内应用.
- 开发的材料有望作为可注射植入物,用于使用无金属CEST-MRI增强癌症检测.
相关概念视频
01:15Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...
01:13Radiological Investigation III: Pulmonary Angiogram and PET Scan
Radiological investigations are paramount in the diagnosis and management of various pulmonary diseases. Two essential investigations are the Pulmonary Angiogram and the Positron Emission Tomography (PET) Scan.
Pulmonary Angiogram
A Pulmonary Angiogram is an invasive procedure involving injecting a contrast medium through a catheter threaded into the pulmonary artery or the right side of the heart to visualize the pulmonary vasculature. Computed Tomography (CT) scans have mainly replaced this...
Pulmonary Angiogram
A Pulmonary Angiogram is an invasive procedure involving injecting a contrast medium through a catheter threaded into the pulmonary artery or the right side of the heart to visualize the pulmonary vasculature. Computed Tomography (CT) scans have mainly replaced this...