可持续的menaquinone-7生产通过生物膜生物反应器的连续发酵
Aydin Berenjian1,2, Ehsan Mahdinia3, Ali Demirci4
1Department of Chemical and Biological Engineering, Colorado State University, Fort Collins, CO, 80523, USA.
Bioprocess and biosystems engineering
|June 12, 2024
概括
这项研究优化了使用Bacillus subtilis natto生物膜反应器的连续发酵,以提高Menaquinone-7 (MK-7) 产量. 稀释率为0.042h-1产生了最高的MK-7生产率,证明了可持续的工业潜力.
科学领域:
- 生物技术是生物技术.
- 微生物发酵 微生物发酵
- 维生素合成 维生素合成
背景情况:
- menaquinone-7 (MK-7) 是一种重要的维生素,通过生物膜形成,主要由Bacillus菌株在细胞外产生.
- 对于MK-7生产的传统批量发酵方法在效率和可持续性方面存在局限性.
研究的目的:
- 开发和优化使用生物膜反应器的连续发酵过程,以提高MK-7的生产.
- 确定最佳的操作参数,特别是稀释率,以最大限度地提高MK-7的产量和生产率.
主要方法:
- 使用一个生物膜反应器,在塑料复合材料支上固定了Bacillus subtilis natto细胞.
- 进行了连续发酵实验,系统地改变稀释速率 (0.007到0.084小时-1).
- 在不同的稀释速率下监测MK-7的生产和生产率,以确定最佳条件.
主要成果:
- 由于稀释速率从0.007小时-1增加到0.042小时-1.1,MK-7的生产率从0.12 mg/L/h增加到0.33 mg/L/h.
- 最佳稀释率为0.042小时−1,导致MK-7的最高生产率为0.33 mg/L/h.
- 较高的0.084小时-1稀释率导致生产率下降 (0.16毫克/升/小时),因为保留时间不足.
结论:
- 生物膜反应器中的持续发酵是MK-7生产的可持续和高效战略.
- 最佳稀释率为0.042小时-1 (相当于48小时的保留时间),最大限度地提高了MK-7的生产力.
- 这些发现支持了持续发酵的工业规模可行性,以提高MK-7产量.
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