在Trichomonas vaginalis细胞外囊泡上调并直接转移粘附因子,促进宿主细胞殖民
Joshua A Kochanowsky1, Portia M Mira1, Samira Elikaee1
1Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA 90095.
概括
阴道的细胞外囊泡 (TvEVs) 介导寄生虫与寄生虫的通信. 来自粘附菌株的TvEV增强了粘附性和聚合在较少粘附菌株中,影响混合感染.
科学领域:
- 寄生虫学的寄生虫学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 阴道 (Trichomonas vaginalis) 是一种常见的性传播寄生虫.
- 由T. vaginalis分泌的细胞外囊泡 (TvEVs) 被宿主细胞吸收,但它们在寄生虫-寄生虫相互作用中的作用尚不清楚.
- 多种T. vaginalis菌株的混合感染很常见,并表现出不同的毒性.
研究的目的:
- 研究TvEVs在调解不同T. vaginalis菌株之间的通信中的作用.
- 了解TVEV如何影响混合感染期间的寄生虫聚合和粘附.
主要方法:
- 来自粘附性 (B7RC2) 和不太粘附性 (G3) 的T. vaginalis菌株的光标记的TVEV被用于评估G3寄生虫的吸收.
- 对用B7RC2-EVs预化的G3寄生虫进行了转录基因分析.
- 蛋白质组学和超分辨率显微镜被用来识别转移的粘附因子.
主要成果:
- 来自更粘附的B7RC2菌株的TvEV被G3寄生虫更有效地内化.
- 与B7RC2-EVs的预化增加了G3寄生虫聚合和对宿主细胞的粘附.
- TvEVs上调了寄生虫膜蛋白的表达,包括粘附因子HPB2,并将卡德林类蛋白转移到受体寄生虫表面.
结论:
- 在T. vaginalis种群中,tvEVs作为寄生虫-寄生虫通信的媒介.
- 这种沟通可以改变寄生虫的粘附和聚合,可能会影响混合感染中的病原体.
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