数以百计的抗微生物可以为昆虫肠道共生生物形成选择性屏障
Joy Lachat1, Gaëlle Lextrait1, Romain Jouan1
1Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell, Gif-sur-Yvette 91198, France.
概括
昆虫肠道中的抗微生物 (AMP) 形成了选择性屏障,但共生生物产生了耐药性. 这些AMP也可能在殖民后调节宿主-微生物相互作用.
科学领域:
- 微生物学 微生物学
- 昆虫生物学 昆虫生物学
- 生态生态学 生态生态学
背景情况:
- 肠道微生物群的空间组织对生态系统功能至关重要,但人们对其了解甚少.
- 像Riptortus pedestris这样的昆虫表现出独特的肠道微生物生物地理,在前后区域有特定的社区.
研究的目的:
- 为了研究肠道微生物群组织背后的机制在Riptortus pedestris.
- 为了确定后部中肠共生体如何抵抗抗微生物 (AMP).
主要方法:
- 转位子测序 (Tn-seq) 用于确定 *Caballeronia insecticola* 中AMP耐药性的遗传决定因素.
- 对抗微生物 (AMP) 活性和细菌耐药机制的分析.
主要成果:
- 中肠后部产生许多具有抗微生物活性的Crypt特定的囊丰富 (CCR).
- 肠道共生体拥有对CCRs的抵抗通路,这对于殖民后部中肠道至关重要.
- 细菌在殖民后分化为CCR敏感状态,这表明CCRs有额外的作用.
结论:
- 抗微生物 (AMP) 作为选择性屏障,塑造肠道微生物群的组成.
- 细菌对AMP的耐药性对于共生至关重要.
- 在肠道微生物群控制和宿主微生物相互作用中,CCR可能具有双重作用.
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