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Yuan Wang¹, Jin Qu¹, Chuxiao Xiong¹

¹Department of Biomedical Engineering, City University of Hong Kong, Kowloon, Hong Kong Special Administrative Region 999077, China.

Proceedings of the National Academy of Sciences of the United States of America

|June 12, 2024

概括

一种基于水凝的新型设备可以有效地通过皮肤传递和电穿孔DNA用于癌症免疫治疗. 这种方法显著增强了抗原特异性免疫反应,并且在减少剂量时抑制了瘤生长.

科学领域:

生物医学工程生物医学工程
免疫学免疫学免疫学
材料科学材料科学材料科学

背景情况:

核酸疫苗有希望,但需要有效的体内基因传递.
目前用于DNA传递和电穿孔的方法在有效性和局部向方面存在局限性.
开发先进的输送系统对于增强癌症免疫疗法至关重要.

研究的目的:

开发一种基于水凝的有机电子设备 (μEPO),用于通过皮肤输送核酸和体内电穿孔.
为了实现癌症免疫疗法的皮下抗原呈现细胞 (APC) 的高效的一步DNA转染.
证明μEPO技术在激活抗原特异性免疫反应和抑制瘤生长方面的有效性.

主要方法:

用含有干燥DNA的微针电极制造基于水凝的有机电子设备 (μEPO).
用μEPO装置将DNA通过皮肤传递和电穿孔到皮肤细胞中的应用.
在使用卵蛋白 (OVA) -DNA的癌症疫苗模型中评估免疫反应和抗瘤疗效.

主要成果:

EPO装置在体内实现了表皮层和皮肤上层超过50%的细胞的传染.
使用μEPO通过皮肤输送OVA-DNA诱导了强大的细胞和幽默免疫反应,包括IFN-γ+细胞毒性T淋巴细胞.
与肌内注射相比,μEPO方法显示了超过10倍的剂量节约,并有效抑制了瘤的生长.

结论:

EPO装置提供了一种高效和局部化的方法,用于通过皮肤输送DNA和电穿孔.
这项技术通过增强抗原特异性免疫力,显著提高了用于癌症免疫治疗的DNA疫苗接种策略.
EPO技术为基于核酸的疗法提供了传统注射方法的有希望的替代方案.

关键词:

接种DNA疫苗接种DNA疫苗癌症免疫疗法免疫疗法水凝电子设备的电子设备在活体中,电穿孔.通过皮肤传递基因

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