广泛的排毒NO还原酶在无氧状态下给N2O赋予了独特的同位素指纹
Renée Z Wang1, Zachery R Lonergan2, Steven A Wilbert2
1Division of Geological and Planetary Sciences, Caltech, Pasadena, CA 91101.
血球蛋白 (Fhp) 是一种细菌酶,产生具有独特同位素特征的氧化 (N2O). 这一发现表明,Fhp是各种环境中N2O的重要,以前被忽视的来源.
科学领域:
- 环境微生物学 环境微生物学
- 生物地质化学生物地质化学
- 同位素地球化学 同位素地球化学
背景情况:
- 氧化 (N2O) 是一种强大的温室气体,具有多种生物和非生物来源.
- 同位素位点偏好 (SP) 值区分由细菌和真菌氧化减少酶 (NOR) 生产的N2O.
- 血球蛋白 (Fhp),一种细菌的NO还原酶,在N2O生物地质化学中基本被忽视了.
研究的目的:
- 研究黄血球蛋白 (Fhp) 在N2O生产中的生态化学作用.
- 为了确定 Fhp.产生的 N2O 的同位素特征.
- 评估 Fhp 对环境 N2O. 的贡献.
主要方法:
- 使用了具有明显的 Fhp 和 NOR 酶表达的 * Pseudomonas aeruginosa * 菌株.
- 在体内测量N2O同位素位置偏好 (SP) 值在不同的生理条件下.
- 比较了Fhp主导,NOR主导和野生型菌株的N2O SP值.
主要成果:
- Fhp产生N2O,其明显的无氧SP值约为+10‰.
- 来自Fhp和NOR酶的N2O SP值差异超过10‰.
- 细胞Fhp:NOR比率,受生理状态的影响,控制网N2O SP生物签名.
- 在测序的细菌基因组中,Fhp同类几乎比NOR同类多出四倍.
结论:
- Fhp是环境N2O的重要贡献者,它具有独特的同位素特征.
- Fhp的丰富性和独特的同位素指纹需要修订的框架来归因N2O的生物来源.
- 了解FHP的作用对于在自然生态系统中准确的N2O流量评估和诊断人类感染至关重要.
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