加强临床决策:Sysmex UF-5000作为儿童细菌性尿路感染查工具
Ping Liu1,2, Chuanwei Ban1,2, Juan Wang1,2
1Clinical Laboratory, Children's Hospital Affiliated to Shandong University, Jinan, China.
PloS one
|June 12, 2024
概括
赛斯麦克斯UF-5000流细胞计有效地选小儿尿样以检测尿路感染 (UTI),识别消极培养和显著的细菌生长. 这种快速测试有助于减少不必要的培养,并指导早期治疗决策.
科学领域:
- 临床诊断 临床诊断 临床诊断
- 儿童传染病 儿童传染病
- 微生物学 微生物学
背景情况:
- 儿童尿路感染 (UTI) 需要快速查测试,以告知临床决策.
- 目前的方法往往需要长时间的尿液培养,延迟诊断和治疗.
- 需要有效的工具来避免不必要的培养,并加快患者的护理.
研究的目的:
- 评估Sysmex UF-5000流细胞计作为儿童患者尿路感染的查工具.
- 与尿液培养结果相比,评估分析仪在检测白细胞 (WBC) 和细菌 (BACT) 方面的性能.
- 确定UF-5000在识别负培养和显著细菌生长方面的实用性.
主要方法:
- 对2020年1月至2023年9月期间收集的4445个儿科尿样进行分析.
- 使用Sysmex UF-5000流细胞计测量尿液WBC和BACT计数.
- 将UF-5000检测结果与标准尿液培养结果进行比较,以评估诊断准确性.
主要成果:
- 阳性培养物的最佳截止值是BACT在92.2/μL (AUC:0.944) 和WBC在40.8/μL (AUC:0.863).
- 7.8 /μL的BACT切断值实现了0.9%的错误负率,从而避免了28.1%的不必要的培养.
- 在UF-5000中,Gram阴性 (90.3%) 比Gram阳性 (86.8%) 细菌的稳定性更高,对显著的细菌生长具有更高的一致性.
结论:
- 赛斯麦克斯UF-5000显示为选儿科尿液培养的前景,识别负面结果和显著的细菌生长.
- 分析仪有助于减少不必要的培养物,并可以指导经验性抗生素治疗,特别是在格拉姆阴性尿路感染.
- 性能可能因研究人群而异,但Gram typing能力提高了临床决策.
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