该MdHSC70-MdWRKY75模块通过调节热冲击因子基因的表达来调节果基底热耐受性
Zhijun Zhang1, Chao Yang1, Jing Xi1
1State Key Laboratory for Crop Stress Resistance and High-Efficiency Production/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A & F University, Yangling 712100, Shaanxi, China.
The Plant cell
|June 12, 2024
概括
果公司种植果植物.
科学领域:
- 植物科学 植物科学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 热应激对果生长和水果发育有负面影响.
- WRKY转录因子在果耐热性中的作用在很大程度上是未知的.
研究的目的:
- 研究MdWRKY75在果耐热性中的作用.
- 阐明果中热应激反应背后的分子机制.
主要方法:
- 在果植物中,基因过度表达和沉默.
- RNA测序 (RNA-seq) 和定量的PCR.
- 酵母单杂交,电泳性移动性转移和双露西法酶试验.
- 酵母二杂交,分裂光酶和双分子光补充试验.
主要成果:
- MdWRKY75积极调节果的基本耐热性.
- MdWRKY75直接激活热冲击因子基因 (MdHsfs) 的表达.
- MdHSC70与MdWRKY75相互作用,并负面调节热应激反应.
结论:
- 一个涉及MdHSC70,MdWRKY75和MdHsfs的分子模块调解了果对热应激的反应.
- 这项研究增强了对果中WRKY转录因子调节的热耐受性的理解.
相关概念视频
Responses to Heat and Cold Stress
13.4K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
13.4K
Thermoregulation
964
The human body has a sophisticated thermoregulation system that employs negative feedback mechanisms to maintain an optimal core temperature. When the core temperature drops, peripheral and central thermoreceptors send signals to the hypothalamus, activating the heat-promoting center. This center triggers several responses aimed at increasing the core temperature. First, vasoconstriction reduces the flow of warm blood from internal organs to the skin so that the heat is not lost from the skin,...
964
Background and Environment Affect Phenotype
6.5K
Although the genetic makeup of an organism plays a major role in determining the phenotype, there are also several environmental factors, such as temperature, oxygen availability, presence of mutagens, that can alter an organism’s phenotype.
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
6.5K
Master Transcription Regulators
6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K
Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K
Thermosensation
30.4K
Peripheral thermosensation is the perception of external temperature. A change in temperature (on the surface of the skin and other tissues) is detected by a family of temperature-sensitive ion channels called Transient Receptor Potential, or TRP, receptors. These receptors are located on free nerve endings. Those detecting cold temperatures are closer to the surface of the skin than the nerve endings detecting warmth. These thermoTRP channels, while temperature selective, have relatively...
30.4K


