相关实验视频

Updated: Jun 24, 2025

Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells

Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells

Published on: July 22, 2013

硫化维持了线粒体平衡,并通过SQR在热应激下调节甘酸生物合成,在甘白中

Jiaolei Shangguan¹, Tao Wu¹, Li Tian¹

¹Sanya Institute of Nanjing Agricultural University, Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, Microbiology Department, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, PR China.

|June 12, 2024

概括

硫化 (H2S) 通过维护线粒体功能来保护Ganoderma lucidum免受热应激. 这种气态发射器激活关键酶,维持细胞平衡,减少不必要的甘酸生物合成.

科学领域:

菌类学菌类学是指菌类学.
生物化学生物化学
环境微生物学环境微生物学

背景情况:

硫化 (H2S) 是一种具有多种生理作用的信号分子.
已经证明H2S可以减轻热引起的甘酸 (GA) 生物合成在甘 lucidum中的下降.
在热应激下G. lucidum中H2S作用的精确机制尚未完全理解.

研究的目的:

阐明G. lucidum中H2S对热应激反应的生理效应和分子机制.
研究H2S在维护线粒体功能和调节热应激下GA生物合成中的作用.

主要方法:

药理学和基因操纵细胞内H2S水平.
评估线粒体参数:膜潜力 (MMP),DNA复制数 (mtDNAcn),氧耗率和ATP含量.
酶活性测定硫化氧化还原酶 (SQR) 和复合III (Com III).
对GA生物合成途径的分析.

主要成果:

在G. lucidum. 中,热应激减少了MMP和mtDNAcn.
外源H2S在热应激下增加了MMP,mtDNAcn,氧气消耗和ATP水平.
发现H2S可以激活SQR和Com III,从而保持线粒体平衡.
通过SQR调解H2S对GA生物合成的负调节,并且可能经历持续硫化.

结论:

H2S在保护G. lucidum免受热引起的线粒体损伤方面发挥着至关重要的作用.
通过H2S激活SQR和Com III是维持在压力下线粒体功能的关键.
涉及SQR的H2S信号提供了一个新的机制来调节GA生物合成,以应对环境变化.

关键词:

甘酸是一种甘酸.这种植物叫做Ganoderma lucidum.硫化是一种硫化.线粒体的恒常性平衡.硫化物-氨酸氧化降解酶是硫化物-氨酸氧化降解酶.

更多相关视频

Assessing Mitochondrial Function in Sciatic Nerve by High-Resolution Respirometry

Assessing Mitochondrial Function in Sciatic Nerve by High-Resolution Respirometry

Published on: May 5, 2022

Experimental Protocol for Detecting Mitochondrial Function in Hepatocytes Exposed to Organochlorine Pesticides

Experimental Protocol for Detecting Mitochondrial Function in Hepatocytes Exposed to Organochlorine Pesticides

Published on: September 16, 2020

相关概念视频

Overview of Metabolism

Overview of Metabolism

Living cells constantly carry out various chemical reactions which are necessary for their proper functioning. These reactions are interlinked to one another via multiple pathways. The collection of these chemical reactions is known as metabolism.
Plant Metabolism
Sunlight, the primary source of energy in plants, is first absorbed by the chlorophyll pigments present in their leaves. Plants then use this energy to carry out photosynthesis, where water is oxidized into oxygen and carbon dioxide...

Responses to Heat and Cold Stress

Responses to Heat and Cold Stress

Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.

Mitochondrial Membranes

Mitochondrial Membranes

A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...