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一种植物NLR的氧化介导自抑制和辅因子结合

Shoucai Ma^1,2, Chunpeng An³, Aaron W Lawson³

¹School of Life Sciences, Westlake University, Institute of Biology, Westlake Institute for Advanced Study, Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, China. mashoucai@xhlab.ac.cn.

|June 12, 2024

概括

植物免疫受体,核酸结合丰富的重复蛋白 (NLR),可以通过二分化自我抑制. 伊诺西酸盐与这些受体结合,调节植物免疫力并防止自身免疫力.

科学领域:

植物免疫力
植物防御的分子机制
蛋白质的结构和功能

背景情况:

核酸结合丰富的重复蛋白 (NLR) 对于植物免疫反应至关重要,识别病原体的作用.
无法控制的NLR活动可能导致自身免疫,强调需要严格监管.
细胞死亡2 (NRC2) 所需的植物NLR是构成性表达的,但仍然不活跃,其调节机制尚不清楚.

研究的目的:

阐明Solanum lycopersicum NRC2 (SlNRC2) 自抑制和激活的机制.
调查化和内酸盐在SlNRC2调控中的作用.

主要方法:

低温电子显微镜 (cryo-EM) 用于确定SlNRC2寡合物的结构.
质谱测试以确认因诺酸盐结合.
位点定向突变,以评估已识别的接口和结合位点的功能.

主要成果:

在较高度下,SlNRC2形成二聚体,四聚体和更高阶的寡聚体,采用无活性构造.
氧化稳定了非活性状态,并阻止了活性形式的组装.
冷EM检测显示了与SlNRC2的C端域结合的伊诺西六酸盐 (IP6) 或五酸盐 (IP5).
突变破坏了二分化接口或异醇结增强了植物免疫力和细胞死亡.

结论:

氧化和内酸盐作为NRC2激活的负调节剂,保持其无活性状态.
伊诺西酸盐可以作为NRC蛋白的辅助因子,调节植物的免疫反应.
了解这些调节机制是控制植物免疫力和预防自身免疫力的关键.

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