由大脑衍生的神经营养因子载入的低温敏感脂质体作为药物输送系统,用于修复受细胞损伤
Xiaoyi Huang1, Min Li2, Maria Isabel Martinez Espinoza1
1Dipartimento di Chimica, Materiali ed Ingegneria Chimica "G. Natta". Politecnico di Milano, Via Mancinelli 7, 20131 Milan, Italy.
International journal of pharmaceutics
|June 12, 2024
概括
向性脂质体提供来自大脑的神经营养因子 (BDNF),以修复脏受细胞损伤. 这种新疗法通过减少蛋白尿和改善组织学来治疗球性疾病具有前景.
科学领域:
- 腎臟病學 (nephrology) 是一種醫學.
- 生物技术是生物技术.
- 药物输送系统 药物输送系统
背景情况:
- 细胞对功能至关重要,也是治疗病的关键标.
- 大脑衍生神经营养因子 (BDNF) 显示出对细胞损伤的治疗潜力,但其短半衰期限制了对肠道给药.
- 低温敏感脂质体 (LTSL) 提供了一种稳定,有针对性的活性BDNF输送方法.
研究的目的:
- 加强LTSL设计,以实现有效的BDNF封装和有针对性的释放到 podocytes.
- 通过联来改善LTSL稳定性和生物活性,以针对特定的质内皮细胞.
- 为了评估BDNF载荷的LTSL在体内和体内治疗球性疾病的疗效.
主要方法:
- 开发了BDNF载荷的LTSL,增强了封装和稳定性.
- 结合向 (cRGD in vitro,HITSLLS in vivo) 用于特定的细胞吸收.
- 在共同培养模型中评估了细胞修复,白蛋白透性和细胞骨损伤.
- 在体内评估脏积累,减少蛋白尿和组织学变化.
主要成果:
- 载有BDNF的LTSL在体外修复了受细胞细胞骨损伤,并降低了专蛋白的透性.
- cRGD结合改善了内皮细胞的向和吸收,在热反应释放时增强了治疗效果.
- 在体内研究表明脏积累的目标LTSL,减少蛋白尿和改善脏组织学.
- 该HITSLLS在体内促进了质内皮细胞的特定吸收.
结论:
- 优化的BDNF载荷的LTSL配方显示出有效的向输送和球细胞疾病的治疗潜力.
- 类结合LTSL通过控制BDNF释放来增强准特异性和治疗结果.
- 这种方法为恢复细胞功能和治疗脏疾病提供了一个有希望的策略.
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