通过生物对等点击反应对病毒样颗粒的表面功能化,以增强细胞特异向
Chavee Laomeephol1, Supannikar Tawinwung2, Koramit Suppipat3
1Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand; Center of Excellence in Biomaterial Engineering in Medical and Health, Chulalongkorn University, Bangkok 10330, Thailand.
International journal of pharmaceutics
|June 12, 2024
概括
具有亚基组的工程病毒样颗粒 (VLPs) 显示了对T细胞的增强向. 生物直角点击化学允许通用功能,用于精确的药物输送和改善细胞吸收.
科学领域:
- 生物技术是生物技术.
- 纳米医学是一种纳米医学.
- 免疫学 免疫学 免疫学
背景情况:
- 纳米颗粒的表面功能化对于有针对性的药物输送至关重要.
- 病毒样粒子 (VLP) 为治疗提供了一个多功能平台.
- 细胞内向T细胞的输送是具有挑战性的,因为外源物质的吸收率低.
研究的目的:
- 为增强T细胞向而设计化物功能化的VLP.
- 为了利用生物对角点击化学用于特定的VLP结合.
- 改善治疗分子向T细胞的细胞内传递.
主要方法:
- 在脂质膜上重组生成HIV-1Gag VLP与亚基.
- 生物对等点击反应用于将环基因载体分子与VLPs结合.
- 共同培养系统评估CD3阳性T细胞对亚酸VLP的选择性吸收.
主要成果:
- 亚酸功能化VLP显示T细胞增强吸收,即使没有抗体结合.
- 抗体结合进一步放大了VLP的吸收,证明了协同向.
- 通过使用生物直角点击化学实现了高效和特定的结合.
结论:
- 复合性亚化物功能化VLP是多功能纳米粒子,用于有针对性的输送.
- 生物直角点击化学使VLP的简单和特定修改成为可能.
- 这种方法增强了T细胞的向性,并具有各种生物应用的潜力.
相关概念视频
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