Arid5a使用其核心ARID域的无序扩展来进行不同的DNA和RNA识别和基因调节
Julian von Ehr1, Lasse Oberstrass2, Ege Yazgan3
1Institute for Molecular Biosciences and Biomolecular Resonance Center (BMRZ), Goethe University Frankfurt, Frankfurt, Germany; IMPRS on Cellular Biophysics, Frankfurt, Germany.
The Journal of biological chemistry
|June 12, 2024
概括
研究人员确定了Arid5a的DNA和RNA结合偏好,Arid5a是一种参与基因调节的蛋白质. 围绕ARID域的内在无序区域对于其在DNA和RNA相互作用中的双重功能至关重要.
科学领域:
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
- 生物化学 生物化学
背景情况:
- 含有AT丰富相互作用域 (ARID) 的蛋白质 (Arids) 是一个多样化的DNA结合蛋白家族,参与基因转录和染色体调节.
- 这一家族的成员Arid5a的特定DNA结合偏好尚未在实验中得到定义.
- 已知Arid5a可以通过其DNA结合的ARID域结合mRNA动机进行转录稳定,但缺乏公正的RNA动机识别和其核酸结合能力的清晰剖析.
研究的目的:
- 通过实验来确定Arid5a.的DNA结合偏好.
- 通过使用高通量体外结合试验来识别核心ARID域识别的RNA动机.
- 研究内在无序区域 (IDR) 在Arid5a的核酸相互作用及其整体功能中的作用.
主要方法:
- 以核磁共振 (NMR) 为中心的生物化学,以确定Arid5a的DNA偏好.
- 高通量体外结合测试以确定RNA结合动机.
- 单个核酸分辨率交叉链接和免疫沉,然后进行测序 (iCLIP2),以在转录组中映射Arid5a结合点.
主要成果:
- 该研究确定了Arid5a.的特定DNA结合偏好.
- 通过体外结合,确定了核心ARID域的共识RNA结合动机.
- 全转录组iCLIP2分析显示,Arid5a在参与RNA处理的mRNA前转录中对富含AU的区域的偏好较弱.
- 发现围绕ARID域的内在无序区域调节了DNA结合特异性和亲和力,同时对RNA相互作用至关重要.
结论:
- Arid5a通过其扩展的ARID域表现出双功能基因调节,与DNA和RNA相互作用.
- 在调节核酸相互作用时,内在无序区域的参与似乎是Arid蛋白在染色质-mRNA接口上的一般特征.
关键词:
在 ARID 域名中使用 ARID.在Arid5a中,我们可以使用Arid5a.这是一种DNA结合蛋白质.通过RNA结合-n-Seq (RBNS) 进行.这是一种RNA结合蛋白.电泳运动移动转移试验 (EMSA)基因调节 基因调节 基因调节个人核酸分辨率紫外线交联和免疫沉 (iClip2)本质上是无序的蛋白质.核磁共振 (NMR) 是一种核磁共振技术.更多相关视频
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