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通过miR-145-5p/EGFR轴,circZNF609和circNFIX作为可能通过miR-145-5p/EGFR轴调节质母细胞瘤发病的调节剂

Elham Ghadami¹, Ali Gorji^2,3, Ahmad Pour-Rashidi⁴

¹Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

Scientific reports

|June 12, 2024

概括

循环RNAscircZNF609和circNFIX可能通过海绵miR-145-5p驱动质母细胞瘤,从而导致EGFR表达的增加. 这表明质母细胞瘤多种 (GBM) 治疗的新型治疗标.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

质母细胞瘤 (GBM) 是一种具有不良预后的侵袭性脑癌.
异常表达的循环RNAs (circRNAs) 与GBM瘤发生有关.
在GBM中,circZNF609和circNFIX的特定作用仍然在很大程度上是未知的.

研究的目的:

研究质母细胞瘤中circZNF609,circNFIX,miR-145-5p和EGFR的表达水平和潜在的调节机制.
探索circZNF609和circNFIX通过海绵化miR-145-5p调节EGFR的假设.

主要方法:

量化聚合酶连锁反应 (qPCR) 用于测量circZNF609,circNFIX,miR-145-5p和EGFR的表达.
在质母细胞瘤患者样本和正常大脑组织中分析了表达水平.
评估了基因表达和临床病理特征之间的相关性.

主要成果:

在质母细胞瘤中,circZNF609,circNFIX和EGFR的高调显著.
在质母细胞瘤中,miR-145-5p显著下调.
在miR-145-5p与circZNF609,circNFIX和EGFR的表达之间观察到显著的反相关性.

结论:

CircZNF609和circNFIX可能通过海绵miR-145-5p促进质母细胞瘤,从而导致EGFR表达的增加.
这些发现阐明了GBM病变发生的潜在分子途径.
这一途径代表了质母细胞瘤治疗的潜在治疗点.

关键词:

电路FIX 电路FIX 电路电路ZNF6099 的情况.欧洲农业基金会 (EGFR) 是一个基金.在 miR-145-5p 中使用.质母细胞瘤 (glioblastoma) 是一个

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