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单细胞转录组分析显示,机密素是人类肠内分泌细胞成熟的标志

Franc Hysenaj¹, Michael Lauber¹, Andrea Bast-Habersbrunner²

¹Chair of Experimental Bioinformatics, School of Life Sciences, Technical University of Munich, 85354, Freising, Germany.

Scientific reports

|June 12, 2024

概括

对于肠内分泌细胞 (EECs) 的传统"一个细胞,一个激素"模型已经过时了. 这项研究表明,人类的EECs共同表达多种激素,挑战了既定的分类,并揭示了秘密蛋白 (SCT) 表达标志细胞成熟.

科学领域:

胃肠病学胃肠病学
细胞生物学细胞生物学
内分泌学在内分泌学.

背景情况:

1977年建立的肠内分泌细胞 (EECs) 命名法遵循"一个细胞 - 一种激素"的教条.
这种模型基于单一激素分泌的EEC亚种群进行分类,例如S细胞对秘 (SCT).
最近的研究,特别是在小鼠模型中,已经挑战了这个教条,表明在EEC中激素的共同表达.

研究的目的:

调查"一个细胞 - 一个荷尔蒙"教条在人类初级EECs中的有效性.
用单细胞转录组数据分析人类EEC中的激素表达模式.
重新评估表达秘密素 (SCT) 的细胞的分类.

主要方法:

利用肠道细胞图谱,一个全面的单细胞转录组数据集的人类肠道细胞.
进行了荷尔蒙表达的详细分析,重点是分泌素 (SCT).
对比了初级人类EECs的发现与来自小鼠和有机物衍生的EECs的现有数据.

主要成果:

证实"一个细胞 - 一个荷尔蒙"教条对于人类初级EECs已经过时,表明了显著的荷尔蒙共同表达.
发现秘密素 (SCT) 表达不仅限于一个不同的细胞类型 (S细胞),而是由多个EEC亚群共同产生的.
观察到,与原生细胞相比,成熟的EECs中的秘密蛋白 (SCT) 表达显着更高,这表明它是成熟的标志物.

结论:

由于人体细胞中激素的共同表达广泛存在,传统的EEC分类需要修订.
秘 (SCT) 生产是各种EEC类型之间的协作努力,不仅限于单一的人口.
秘 (SCT) 表达作为肠内分泌细胞成熟的可靠指标.

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