甲状腺诱导顿反应依赖的铁死在Vibrio parahemolyticus中
Jia-Yi Zhang1, Xuan Meng1, Xiao-Lin Zhu1
1School of Food Science and Technology, Shaanxi University of Science and Technology, Xi'an 710021, Shaanxi, China.
Journal of agricultural and food chemistry
|June 12, 2024
概括
蒂莫尔通过诱导铁亡,一种依赖于活性氧物种和铁积累的细胞死亡途径,有效地杀死Vibrio parahemolyticus. 这种天然化合物准费里,为控制海鲜中的这种病原体提供了潜力.
科学领域:
- 微生物学 微生物学
- 生物化学 生化学
- 自然产品化学 自然产品化学
背景情况:
- 蒂摩尔具有广泛的杀菌特性.
- 醇对抗Vibrio parahemolyticus的特定机制在很大程度上仍未被阐明.
- 维亚血解菌是水产品中一个重要的病原体.
研究的目的:
- 为了研究甲基醇对抗Vibrio parahemolyticus的杀菌机制.
- 为了探索蒂摩尔作为控制Vibrio parahemolyticus的天然抑制剂的潜力.
主要方法:
- 定量杀菌试验以确定蒂摩尔的疗效.
- 细胞染色技术用于评估活性氧物种 (ROS),铁积累,脂质过氧化和DNA损伤.
- 蛋白质组分析和分子对接,以确定分子目标和途径.
主要成果:
- 蒂莫尔在150μg/mL时显示出99.9%的杀菌活性对抗Vibrio parahemolyticus.
- 蒂莫尔诱导了芬顿反应依赖的铁亡,由增加的ROS,铁积累和脂质过氧化证明.
- 分子对接和体外测试表明,胸腺醇与费里丁相互作用,促进铁的释放.
结论:
- 蒂莫尔有效地通过诱导铁灭菌来杀死Vibrio parahemolyticus.
- 费里丁被确定为一种潜在的分子标,可以调解由甲基醇诱导的铁亡.
- 这些发现支持开发用于控制Vibrio parahemolyticus的基于乳腺素的天然抑制剂.
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