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由牛乳腺上皮细胞产生的可溶性CD14调节它们对全长LPS的反应

Mégane Védrine¹, Florence B Gilbert¹, Sarah Maman²

¹ISP UMR 1282, INRAE, Université François Rabelais de Tours, Nouzilly, France.

Veterinary research

|June 12, 2024

概括

乳腺上皮细胞分泌可溶性CD14 (sCD14),对于识别大肠杆菌脂多糖 (LPS) 至关重要. 这项研究揭示了sCD14.

科学领域:

兽医免疫学兽医免疫学
牛的传染病牛的传染病
细胞微生物学细胞微生物学

背景情况:

牛乳炎是全球重要的牛病.
乳腺上皮细胞 (MEC) 检测到像大肠杆菌这样的细菌病原体.
来自大肠杆菌的脂聚糖 (LPS) 通过TLR4和CD14受体被MECs识别.

研究的目的:

研究可溶性CD14 (sCD14) 在MEC对LPS的反应中的作用.
为了确定MEC是否有助于牛奶中的sCD14水平.
阐明sCD14在LPS识别和下游信号中的机制.

主要方法:

通过MECs证明CD14分泌.
用全长O-抗原 (LPSS) 对LPS的MEC反应的sCD14调制的研究.
对Myd88独立路径激活的分析.
在MEC中识别LPS诱导的长非编码RNA (lncRNAs).

主要成果:

MECs分泌CD14,有助于牛奶中的sCD14.
sCD14对于LPSS.完全激活Myd88独立途径至关重要.
一些lncRNAs,包括MIR99AHG,在MEC中被LPS激活.

结论:

多国经济体积极为牛奶sCD14池做出贡献.
sCD14对于一个完整的MEC对LPS的反应是不可或缺的.
牛奶sCD14在牛乳炎期间识别大肠杆菌病原体方面发挥着至关重要的作用.

关键词:

埃舍里希亚大肠杆菌 (Escherichia coli) 是一个大肠杆菌.CD14 CD14 CD14 CD14 CD14 CD14 CD14 CD14 CD14 CD14 CD14 CD14 CD14 CD14 CD14 CD14 CD14 CD14 CD14 CD14 CD14 CD14 CD14 CD14 CD14 CD14 CD14 CD14 CD14 CD14 CD14 CD14 CD14 在LPS中使用LPS.哺乳动物上皮细胞这是一种炎症炎症炎症炎症.乳腺炎是一种乳腺炎.

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