相关实验视频

Updated: Jun 24, 2025

Development of Compendium for Esophageal Squamous Cell Carcinoma

Development of Compendium for Esophageal Squamous Cell Carcinoma

Published on: April 12, 2024

皮肤状细胞癌进展的基因表达格局

Tomas Bencomo^1,2, Carolyn S Lee^1,3

¹Stanford Program in Epithelial Biology, Stanford University, Stanford, CA, USA.

The British journal of dermatology

|June 13, 2024

概括

这项研究分析了皮肤癌 (cSCC) 转录组,以绘制进展过程中的基因表达变化. 它确定了关键的基因和涉及的细胞类型,发现基因融合是罕见的,并将紫外线暴露与cSCC发育联系起来.

科学领域:

在瘤学瘤学.
基因组学就是基因组学.
皮肤病学皮肤病学

背景情况:

皮肤状细胞癌 (cSCC) 是一种流行癌症,但之前的RNA测序 (RNA-Seq) 研究存在局限性.
样本规模较小和分析方案多样化阻碍了cSCC研究中发现的可转移性.

研究的目的:

使用元分析创建cSCC进展的综合转录组图.
协调和分析公开可用的RNA-Seq数据,从正常皮肤到cSCC阶段.

主要方法:

从正常皮肤,行动性角质炎 (AK),局部SCC,角质腺瘤 (KA) 和cSCC样本中统一处理全转录组数据.
在协调的数据上进行了差异基因表达,融合检测和细胞类型解卷分析.
利用基因型-组织表达 (GTEx) 数据来识别对紫外线敏感的基因特征.

主要成果:

定义了一组差异表达基因 (DEGs) 的共识,包括在侵袭前阶段的基因.
瘤特异性角质细胞 (TSK) 被确定为DEGs的主要表达体.
cSCC亚型显示出不同的细胞组成;免疫抑制患者的免疫细胞较少.
发现基因融合是cSCC的不常见驱动因素.
一个300个基因的特征将慢性阳光照射和紫外线辐射与cSCC发育联系起来.

结论:

这项研究定义了cSCC进展的基因表达格局.
在cSCC亚型及其临床表型中表现出异质性.
基因融合不是cSCC的常见原因.
确定了与cSCC发育相关的紫外线敏感基因.

更多相关视频

Establishment of a Clinic-based Biorepository

Establishment of a Clinic-based Biorepository

Published on: May 29, 2017

Spatial and Temporal Control of Murine Melanoma Initiation from Mutant Melanocyte Stem Cells

Spatial and Temporal Control of Murine Melanoma Initiation from Mutant Melanocyte Stem Cells

Published on: June 7, 2019

相关概念视频

Tumor Progression

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...

Skin Cancer

Skin Cancer

Skin cancer is a type of cancer that occurs when there is an abnormal growth of skin cells, usually triggered by damage to the DNA within the skin cells. It is primarily caused by exposure to ultraviolet (UV) radiation from the sun or artificial sources like tanning beds. Skin cancer is the most common type of cancer worldwide, and its incidence continues to rise.
Basal Cell Carcinoma (BCC): BCC is the most common type of skin cancer, accounting for about 80% of cases. It typically develops in...

Cancer-Critical Genes II: Tumor Suppressor Genes

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...

The Retinoblastoma Gene

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...