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Circ-Astn1的表达通过激活自细胞来抑制高葡萄糖诱导的内皮原生细胞功能障碍
Shiying Huang1,2, Minjie Xu1,3, Maoquan Li1,3
1Department of Interventional & Vascular Surgery, Tenth People's Hospital of Tongji University, Shanghai, China.
Endocrine research
|June 13, 2024
概括
循环RNAcirc-Astn1保护糖尿病中高葡萄糖诱导的内皮细胞损伤. 它针对miR-138-5p/SIRT5通路,促进自和抑制亡.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 遗传学 遗传学是一种遗传学.
背景情况:
- 糖尿病及其并发症是全球健康面临的重大挑战.
- 循环RNAs (circRNAs) 正在成为各种生物过程中基因表达的关键调节者.
- 在DM的发病过程中,circRNAs的特定作用仍然在很大程度上未被探索.
研究的目的:
- 在高葡萄糖 (HG) 条件下研究内皮原生细胞 (EPC) 中的circRNAs的表达和功能,这些条件与DM相关.
- 阐明 circ-Astn1 在 HG 诱导的内皮细胞功能障碍中的作用背后的分子机制.
主要方法:
- 暴露于HG的EPCs中的circRNAs的高通量测序.
- 生物信息学分析以确定潜在的目标和监管途径.
- 路西法酶记者测定,血管生成分化测定,亡的流细胞计和RT-qPCR来验证发现.
主要成果:
- 在HG条件下,EPCs中的circ-Astn1表达被下调.
- 过度表达circ-Astn1保护的内皮细胞从HG诱导的损伤,包括细胞亡和受损的血管分化.
- 发现circ-Astn1对miR-138-5p和SIRT5都具有向作用,并观察到SIRT5的上调和自促进.
结论:
- 在糖尿病中,circ-Astn1对高葡萄糖诱导的内皮细胞损伤起着保护作用.
- 保护机制包括准miR-138-5p/SIRT5轴,增强SIRT5表达,并促进自.
- circ-Astn1代表了治疗糖尿病血管并发症的潜在治疗标.
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