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从光学连贯显微镜的组织幻灯片图像的数字组织学染色

Sangjin Lee¹, Eunji Lee¹, Hyunmo Yang¹

¹Department of Biomedical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Republic of Korea.

Biomedical optics express

|June 13, 2024

概括

这项研究将光学连贯显微镜 (OCM) 与高级数字病理学的数字染色相结合. 这种新的方法可以快速,无染色的组织病理成像,提高诊断效率.

科学领域:

数字病理学数字病理学
光学成像技术的成像
组织病理学组织病理学

背景情况:

数字病理学从无染色光学成像和数字染色中受益,以简化样本准备和快速获取基因病理信息.
光学连贯显微镜 (OCM) 提供无染色成像,但在组织病理幻灯片观察方面面临局限性.

研究的目的:

引入一种新的方法,将广场OCM与用于组织病理幻灯片成像的数字染色相结合.
为了优化组织学幻灯片的生产,用于数字染色和OCM对比.
通过对传统染色方法进行比较分析,评估联合方法的有效性.

主要方法:

整合广场光学连贯显微镜 (OCM) 与数字染色技术.
优化组织学幻灯片的准备,以提高OCM对比度和数字染色的兼容性.
使用组合技术对各种小鼠组织进行成像.
与传统明亮场显微镜进行比较分析.

主要成果:

通过使用联合广场OCM和数字染色方法,成功对各种小鼠组织进行成像.
优化的幻灯片生产过程为OCM提供了高对比度,并适合数字染色.
对比分析表明,拟议的方法与传统的染色方法相比是有效的.

结论:

广场OCM和数字染色的结合为组织病理成像提供了一个可行的,高效的替代方案.
该研究强调了通过无染色技术改善数字病理学工作流程的潜力.
需要进一步研究数字染色在不同组织和厚度的外观的概括性.

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