触发中的STORM显微镜
Péter Bíró1, Tibor Novák1, Elvira Czvik1
1Department of Optics and Quantum Electronics, University of Szeged, Dóm tér 9, Szeged 6720, Hungary.
Biomedical optics express
|June 13, 2024
概括
在单分子局部化显微镜 (SMLM) 中同步光子ON状态与相机曝光提高了局部化精度. 这种方法提高了平均信号强度,并减少重叠事件,以获得更好的超分辨率成像.
科学领域:
- 生物物理学的生物物理.
- 光学显微镜的使用方法
- 超高分辨率的成像技术
背景情况:
- 标准的单分子定位显微镜 (SMLM) 方法表现出独立的光交换和数据采集.
- 这种独立性导致分子在多个中闪,降低了定位精度并增加了重叠概率.
研究的目的:
- 为了研究在SMLM中同步光体ON状态与相机曝光时间的影响.
- 为了提高定位精度并减少超分辨率显微镜中的重叠事件.
主要方法:
- 采用理论分析和模拟来建模同步的影响.
- 专注于分析点差函数强度和定位精度的变化.
主要成果:
- 同步导致本地化较少,平均总信号高15%.
- 通过这种同步方法,重叠本地化的概率减少了10%.
结论:
- 同步光光开关与相机曝光是一个可行的策略,以提高SMLM性能.
- 这种技术通过提高本地化精度和最小化数据工件来提高图像质量.
相关概念视频
01:22Overview of Microscopy Techniques
10.2K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
10.2K
01:37Super-resolution Fluorescence Microscopy
6.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
6.9K