将金字塔虫耐药基因转移到精英牛豆品种Zaayura中,使用标记器辅助的逆交
Patrick Attamah1, Francis Kusi1, Alexander Wireko Kena2
1CSIR-Savanna Agricultural Research Institute, P.O. Box TL 52, Tamale, Ghana.
Heliyon
|June 13, 2024
概括
通过使用标记器辅助逆交,将两个基因嵌入到首选品种中,从而增强了牛花的耐药性. 改进的线条显示出优越的虫耐药性,为农民提供持久的害虫管理解决方案.
科学领域:
- 农业科学 农业科学
- 遗传学 是一个遗传学.
- 昆虫学 昆虫学是一门学科.
背景情况:
- 牛豆 (Aphis cracivora) 对牛豆作物造成了重大经济损害.
- 杀虫剂用于虫控制在西非是常见的,导致农民支出.
- 害虫生物型和耐药性分解需要持久的耐药性策略.
研究的目的:
- 通过基因金字塔化开发耐虫的牛豆品种,通过基因金字塔化开发具有持久的耐药性.
- 为了将两种防虫基因入农民最喜欢的牛豆品种Zaayura.
主要方法:
- 用标记器辅助逆交 (MABC) 来对抗性基因进行内进.
- 简单序列重复 (SSR) 和单核酸多态 (SNP) 标记被用于前景选择.
- 进行了背景选择和反复的母基因组恢复分析.
主要成果:
- 在ICF2一代中的五个金字塔系将两种虫耐药基因固定在双同卵性主导状态中.
- 重复的母基因组恢复率在72%至93.8%之间,其中一行显示恢复率最高.
- 与人工感染下的捐赠父母相比,金字塔线表现出优越的虫耐药性.
结论:
- 开发出的金字塔形线条具有持久的虫耐药性,可以作为新品种释放.
- 这些线条作为牛育种计划的宝贵遗传资源,旨在增强虫耐药性.
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