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MBPD:为One Health实践提供多种细菌病原体检测管道

Xinrun Yang¹, Gaofei Jiang¹, Yaozhong Zhang¹

¹Laboratory of Bio-Interactions and Crop Health, Jiangsu Provincial Key Laboratory for Organic Solid Waste Utilization, Joint International Research Laboratory of Soil Health, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, National Engineering Research Center for Organic-Based Fertilizers, College of Resources and Environmental Sciences Nanjing Agricultural University Nanjing China.

|June 13, 2024

概括

一个新的多种细菌病原体检测 (MBPD) 管道通过使用全面的16S基因数据库和优化测序区域来准确识别动物,植物和动物病原体来提高生物安全.

科学领域:

微生物学微生物学
生物信息学是一种生物信息学.
基因组学就是基因组学.

背景情况:

细菌病原体对生物安全,环境健康和农业构成重大风险.
目前用于病原体检测的16S rRNA基因测序方法在分类分辨率和数据库适用性方面存在局限性.
先进的评估工具对于有效的细菌病原体控制至关重要.

研究的目的:

开发和验证用于多种细菌病原体检测 (MBPD) 的新型管道.
为了提高16S rRNA基因测序对各种病原体的识别准确性和适用性.
通过全面的病原体监测,支持"一个健康"倡议.

主要方法:

从1986年细菌病原体物种 (72,685个序列) 的全长16S基因的精选数据库的创建.
开发MBPD管道,用于识别动物,植物和动物传染病原体.
在全长和可变区域测序的in silico比较中,确定V3-V4是最佳的.

主要成果:

与现有方法 (16SPIP,MIP) 相比,MBPD管道在病原体检测方面表现优越.
与全长测序相比,V3-V4变量区域对病原体识别具有很高的准确性 (88.37%).
在现实世界样本中,MBPD成功识别了已知的病原体和共同污染剂.

结论:

MBPD管道提供了增强的分类分辨率和更广泛的适用于细菌病原体检测.
优化V3-V4区域的使用提高了病原体识别的效率和准确性.
在"一个健康"框架下,MBPD为综合农业,兽医,医疗和环境监测提供了有价值的工具.

关键词:

16S rRNA 基因测序的基因测序一个健康,一个健康.细菌病原体检测检测细菌病原体检测

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