氧化对头癌细胞的增殖和糖解有多种影响
Paopanga Kokilakanit1, Sittichai Koontongkaew2, Kusumawadee Utispan1
1Oral Biology Research Unit, Faculty of Dentistry, Thammasat University (Rangsit Campus), Khlong Luang, Pathum Thani 12120, Thailand.
Biomedical reports
|June 13, 2024
概括
氧化 (NO) 对头部和部状细胞癌 (HNSCC) 的扩散有双重影响. 低度的NO在某些HNSCC细胞中促进了增殖和糖解,而高度则抑制了生长.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物化学 生化学
背景情况:
- 糖解是一种关键的能量通路,也是癌症的标志.
- 氧化 (NO) 是一种信号分子,它影响癌细胞的行为,包括头部和部状细胞癌 (HNSCC) 的细胞周期和细胞亡.
- 在HNSCC中NO对糖解的具体影响尚不清楚.
研究的目的:
- 为了研究NO对HNSCC细胞增殖的影响.
- 分析NO对HNSCC.中葡萄糖载体 (GLUT) 基因表达的影响.
- 为了评估NO对HNSCC细胞系中关键糖分分析指标的影响.
主要方法:
- 使用的同位素HNSCC细胞系 (HN18/HN17和HN30/HN31) 经过氧化捐赠剂 (二甲基胺NONOate) 处理.
- 通过MTT测定评估细胞增殖,并使用Griess反应剂系统测量NO度.
- 使用RT-qPCR量化GLUT1-4基因表达,并通过色度测定测试测量六基因酶活性和乳酸生产.
主要成果:
- 氧化表明了度依赖的作用:在5-200μM时有增殖作用,在200μM以上度时有抗增殖作用.
- 在HN18细胞中,5μM NO通过上调GLUT1/2表达和增加六酶活性和乳酸盐生产来增强增殖和糖解.
- 在HN17和HN30细胞中,5-20μM的NO促进了增殖和调高了GLUT2/3/4的表达,但没有显著影响糖分解路径.
结论:
- 氧化在不同的HNSCC细胞系上表现出明显的增殖作用.
- 可能通过刺激特定细胞类型 (如HN18) 中的葡萄糖代谢和糖解来增强HNSCC细胞增殖.
- 其他HNSCC细胞系中NO的增殖效应可能涉及非糖解机制,需要进一步调查.
相关概念视频
Nitric Oxide Signaling Pathway
5.0K
Nitric oxide (NO), an inorganic gas, acts as a potent second messenger in most animal and plant tissues. NO diffuses out of the cells that produce it and enters the neighboring cells to generate a downstream response. NO synthase (NOS) catalyzes NO production by the deamination of the amino acid arginine. There are three isoforms of NOS. Endothelial cells have endothelial NOS (eNOS), nerve and muscle cells have neuronal NOS (nNOS), and macrophages produce inducible NOS (iNOS) upon exposure...
5.0K
Cancer Therapies
7.6K
Cancer therapies are various modes of treatment, such as surgery, radiation therapy, and chemotherapy that are administered to cancer patients.
However, cancer treatments can pose several challenges, as therapies used to kill cancer cells are generally also toxic to normal cells. Moreover, cancer cells mutate rapidly and can develop resistance to chemical agents or radiation therapy. Besides, all types of cancer cells may not respond to the same therapy. Some cancer cells respond to one...
However, cancer treatments can pose several challenges, as therapies used to kill cancer cells are generally also toxic to normal cells. Moreover, cancer cells mutate rapidly and can develop resistance to chemical agents or radiation therapy. Besides, all types of cancer cells may not respond to the same therapy. Some cancer cells respond to one...
7.6K
Adaptive Mechanisms in Cancer Cells
5.7K
Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
5.7K
Electron Transport Chain: Complex I and II
12.9K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
ROS generation is regulated and maintained at moderate levels necessary...
12.9K
Antianginal Drugs: Nitrates and β-Blockers
571
In cardiovascular health, antianginal drugs combat angina pectoris — a condition marked by chest pain owing to diminished blood flow to the heart.
Organic nitrates, such as nitroglycerin, play a pivotal role. Once metabolized, they liberate nitric oxide, a molecular marvel. Nitric oxide triggers guanylyl cyclase and augments cGMP production. This biochemical cascade orchestrates the relaxation of vascular smooth muscles, ushering in vasodilation and enhancing coronary blood flow....
Organic nitrates, such as nitroglycerin, play a pivotal role. Once metabolized, they liberate nitric oxide, a molecular marvel. Nitric oxide triggers guanylyl cyclase and augments cGMP production. This biochemical cascade orchestrates the relaxation of vascular smooth muscles, ushering in vasodilation and enhancing coronary blood flow....
571
Mechanism of Angiogenesis
5.4K
Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
5.4K


