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相关概念视频

Nucleic Acid Structure01:25

Nucleic Acid Structure

6.1K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
6.1K
Molecular Models02:00

Molecular Models

38.2K
Physical models representing molecular architectures of chemical compounds play essential roles in understanding chemistry. The use of molecular models makes it easier to visualize the structures and shapes of atoms and molecules.
38.2K
Nucleic acids02:43

Nucleic acids

161.6K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
161.6K
Nucleic Acids and Nucleotides01:20

Nucleic Acids and Nucleotides

9.0K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and have instructions for its functioning. The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA).
Deoxyribonucleic Acid (DNA)
DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and the organelles such as chloroplasts and mitochondria....
9.0K
RNA Structure01:19

RNA Structure

4.8K
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
4.8K
DNA as a Genetic Template02:05

DNA as a Genetic Template

21.9K
Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
21.9K

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相关实验视频

Updated: Jun 24, 2025

Analyzing and Building Nucleic Acid Structures with 3DNA
16:24

Analyzing and Building Nucleic Acid Structures with 3DNA

Published on: April 26, 2013

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用FRET指导的核酸建模.

Fabio D Steffen1, Richard A Cunha1, Roland K O Sigel1

  • 1Department of Chemistry, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland.

Nucleic acids research
|June 13, 2024
PubMed
概括

这项研究将单分子弗斯特共振能量转移 (FRET) 与计算建模相结合,绘制RNA结构动态图. 该方法通过对实验FRET数据进行过模型来完善RNA结构预测,从而增强机理学理解.

科学领域:

  • 生物物理学的生物物理.
  • 计算生物学 计算生物学
  • 结构生物学 结构生物学

背景情况:

  • RNA的功能多样性源于形状异质性.
  • 绘制核酸组合中的结构过渡的映射对于理解功能至关重要.
  • 单分子光谱学和计算建模提供了互补的方法.

研究的目的:

  • 开发一个框架,使单分子Förster共振能量转移 (FRET) 测量与计算建模相协调.
  • 用FRET数据来过新的RNA结构预测集.
  • 改进对核酸结构动态和相互作用的机制理解.

主要方法:

  • 单分子FRET实验与分子动力学模拟和新结构预测 (Rosetta) 的整合.
  • 使用全原子或隐性光体建模的FRET实验的in silico复制.
  • 应用可访问的接触量作为结构预测的后期评分方法.

主要成果:

  • 通过反驳不兼容的模型,证明了FRET在过RNA结构预测组合中的实用性.
  • 在DNA上成功对FRET辅助建模方法进行了基准测试,并在动态 рибо开关上进行了验证.
  • 用FRET坐标对四向连接组件进行了总结,复制了一个带丝切换器的全球折叠.

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Structure-Based Simulation and Sampling of Transcription Factor Protein Movements along DNA from Atomic-Scale Stepping to Coarse-Grained Diffusion
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Structure-Based Simulation and Sampling of Transcription Factor Protein Movements along DNA from Atomic-Scale Stepping to Coarse-Grained Diffusion

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3D Printing of Biomolecular Models for Research and Pedagogy
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3D Printing of Biomolecular Models for Research and Pedagogy

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相关实验视频

Last Updated: Jun 24, 2025

Analyzing and Building Nucleic Acid Structures with 3DNA
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Analyzing and Building Nucleic Acid Structures with 3DNA

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Structure-Based Simulation and Sampling of Transcription Factor Protein Movements along DNA from Atomic-Scale Stepping to Coarse-Grained Diffusion
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Structure-Based Simulation and Sampling of Transcription Factor Protein Movements along DNA from Atomic-Scale Stepping to Coarse-Grained Diffusion

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3D Printing of Biomolecular Models for Research and Pedagogy
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3D Printing of Biomolecular Models for Research and Pedagogy

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结论:

  • 计算光谱学提高了动态结构组合的可解释性.
  • 开发的管道改善了对核酸相互作用的机制理解.
  • 这种综合方法为研究RNA结构和动态提供了强大的工具.