MtsABC吸收的有助于人类病原体A组链球菌的致病因子通过抵抗宿主的营养免疫防御来抵抗
Nishanth Makthal1,2, Subhasree Saha1,2, Elaine Huang1,2
1Center for Molecular and Translational Human Infectious Diseases Research, Houston Methodist Research Institute, Houston, Texas, USA.
Infection and immunity
|June 13, 2024
概括
甲组链球菌 (GAS) 使用MtsABC吸收系统来逃避宿主免疫防御,特别是calprotectin (CP),并促进感染. 这种获取对于气体毒性和在唾液等营养有限的环境中生存至关重要.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 病原发生和发病的过程.
背景情况:
- 宿主免疫因子如calprotectin (CP) 隔离必要的金属,影响病原体的生长.
- 甲型链球菌 (GAS) 的发病因子受到的竞争的影响,但 (Mn) 的作用尚不清楚.
研究的目的:
- 调查 (Mn) 获取系统在GAS逃避宿主免疫力中的作用.
- 为了确定CP介导的Mn封存对GAS感染控制的贡献.
主要方法:
- 使用了体外和体内研究,包括基因失活和动物感染模型 (WT和CP-/-小鼠).
- 评估了人类唾液中的细菌存活率和依赖Mn的超氧化物脱酶的功能.
主要成果:
- 气体编码的MtsABC被确定为一个关键的Mn吸收系统,以规避CP强加的Mn稀缺.
- 缺乏Mn,由于MtsC无活化或CP缺失,损害了GAS Mn-依赖的超氧化物脱酶活性.
- 在WT小鼠中,MtsABC对GAS毒性至关重要,但在CP-/-小鼠中没有,突出显示了体内相互作用.
- 通过MtsABC介导的Mn获取对于Mn-scant人类唾液中的GAS存活至关重要.
结论:
- MtsABC进口系统是GAS克服宿主强制金属封存的关键机制.
- MtsABC代表了GAS感染的潜在治疗或预防标.
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