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聚氨酸涂层纸张电极用于对NADH的敏感和选择性检测

Supriya Jain¹, Joseph Sonia², Sharmila Prashanth²

¹Polymer Nanobiomaterial Research Laboratory, Smart Materials and Devices, Yenepoya Research Centre, Yenepoya (Deemed to be University), Mangalore 575 018, India.

Langmuir : the ACS journal of surfaces and colloids

|June 13, 2024

概括

一个新的聚氨酸改性纸质电极 (PMPE) 提供了降低尼古丁胺胺氨基二核酸 (NADH) 的灵敏检测. 这种一次性传感器对诊断NADH异常和生物燃料电池具有前景.

科学领域:

电化学电化学电化学
生物材料科学生物材料科学
传感器技术传感器技术

背景情况:

降低尼古丁胺胺二核酸 (NADH) 检测对于诊断生理障碍至关重要.
传统的电极面临着诸如缓慢的电子转移和污染等挑战,限制了NADH检测.
纸质电极为生物分子传感提供了一个灵活,一次性和敏感的平台.

研究的目的:

开发一种导电聚改性纸电极,用于高效的NADH检测.
为了克服传统电极在NADH传感中的局限性.
探索生物燃料电池中开发的传感器的潜力.

主要方法:

通过对l-tyrosine N-carboxy anhydride的环开聚合合成聚氨酸 (PTyr).
通过用PTyr进行滴涂,对纸质电极 (PE) 进行修改,以制造PTyr修改的纸质电极 (PMPE).
使用PMPE的电化学表征和NADH检测.

主要成果:

该PMPE表现出优异的电化学特性,并促进了低电位 (576mV) 的NADH电氧化.
对NADH的线性检测范围为25145μM,低检测极限为0.340μM.
传感器表现出高选择性,没有甲酸干扰,并且具有良好的电催化活性.

结论:

经过PTyr修改的纸质电极是NADH检测的敏感和选择性平台.
开发的传感器为NADH监测提供了比传统电极的优势.
由于其电催化性质,PMPE在生物燃料电池中显示出潜在的应用.

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