相关实验视频

Updated: Jun 24, 2025

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells

Published on: May 22, 2014

糖尿病伤口角质细胞诱导巨细胞JMJD3-介导的Nlrp3通过IL-1R信号表达

Sonya J Wolf¹, Christopher O Audu¹, Jadie Y Moon¹

¹Section of Vascular Surgery, Department of Surgery, University of Michigan, Ann Arbor, MI.

|June 13, 2024

概括

糖尿病伤口角质细胞通过IL-1α发出信号,在巨细胞中增加JMJD3,激活NLRP3炎症酶并损害伤口愈合. 准这种途径可能会改善糖尿病伤口的修复.

科学领域:

伤口愈合治愈伤口愈合
免疫学免疫学免疫学
分子生物学分子生物学

背景情况:

巨细胞 (Mφ) 的可塑性对伤口修复至关重要,但在2型糖尿病中,Mφs仍然炎症,阻碍愈合.
在糖尿病伤口Mφs中观察到NLRP3炎症酶活性增加,但调节机制尚不清楚.

研究的目的:

阐明驱动糖尿病伤口愈合期间巨细胞NLRP3炎症酶激活的分子机制.
确定状细胞衍生信号和表观遗传修饰剂在调节Mφ NLRP3表达中的作用.

主要方法:

研究了糖尿病与非糖尿病伤口角质细胞中的IL-1α表达.
检查了Mφ NLRP3诱导中的IL-1受体介导信号.
评估了JMJD3表达及其在Nlrp3转录激活中通过H3K27me3.3的作用.
利用特定于骨髓细胞的JMJD3缺乏的小鼠 (Jmjd3f/flyz2Cre+) 在体内研究Mφ介导的Nlrp3表达.

主要成果:

糖尿病伤口角质细胞过度表达IL-1α,从而通过IL-1受体信号传递诱导Mφ Nlrp3的表达.
基因组脱甲基酶JMJD3在Mφs上调并由IL-1α诱导,导致Nlrp3的转录激活.
骨髓细胞中的JMJD3在糖尿病伤口愈合期间对Mφ介导的Nlrp3表达至关重要.
皮细胞衍生的IL-1α和IL-1R信号驱动NLRP3炎症酶在Mφs中的过度活跃,在非愈合的糖尿病伤口中.

结论:

在糖尿病伤口愈合过程中,状细胞衍生的IL-1α信号激活了Mφs中的NLRP3炎症酶.
作为一个关键的表观遗传调节剂,JMJD3在Mφs.中调解IL-1α诱导的Nlrp3表达.
IL-1α/IL-1R/JMJD3轴代表了通过调节Mφ炎症酶活性来改善糖尿病伤口愈合的潜在治疗目标.

更多相关视频

Protocol to Create Chronic Wounds in Diabetic Mice

Protocol to Create Chronic Wounds in Diabetic Mice

Published on: September 25, 2019

Come to the Light Side: In Vivo Monitoring of Pseudomonas aeruginosa Biofilm Infections in Chronic Wounds in a Diabetic Hairless Murine Model

Come to the Light Side: In Vivo Monitoring of Pseudomonas aeruginosa Biofilm Infections in Chronic Wounds in a Diabetic Hairless Murine Model

Published on: October 10, 2017

相关概念视频

The JAK-STAT Signaling Pathway

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...

T Cell Types and Functions

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...