基于化的深溶解剂可以抑制属于T4基因型的病原性Acanthamoeba castellanii
Noor Akbar1,2, Amir Sada Khan3,4, Ruqaiyyah Siddiqui5,6
1Research Institute of Medical and Health Sciences, University of Sharjah, 27272, Sharjah, United Arab Emirates. noor@gmail.com.
Folia microbiologica
|June 13, 2024
概括
基于化的深度浸泡溶剂显示出强烈的抗菌活性,可以对抗Acanthamoeba castellanii. DES2表现出显著的氨杀菌和抗粘附作用,而DES3抑制了囊的脱离,表明了治疗潜力.
科学领域:
- 生物化学 生物化学
- 微生物学 微生物学
- 材料科学 材料科学 材料科学
背景情况:
- 病原性自由生活的虫,如Acanthamoeba castellanii,对健康构成重大风险,包括角膜炎和粒状虫脑炎.
- 目前对阿坎萨摩巴菌感染的治疗选择有限,并且经常伴有毒性.
- 深度环氧溶剂 (DES) 是一种具有可调节性质的新型离子流体,在各种生物医学应用中显示出潜力.
研究的目的:
- 调查基于化的新型深溶剂对致病性阿坎萨摩巴 (Acanthamoeba castellanii) (T4基因型) 的抗菌疗效.
- 评估这些DES配方对氨基粘附,脱离和它们对人类大脑内皮细胞的细胞毒性的影响.
主要方法:
- 通过使用三四二化和不同的键捐赠剂 (阿司匹林,多德卡诺酸,4-三-丁酸) 合成了三种疏水性深溶剂 (DES1,DES2,DES3).
- 进行了氨基杀菌性,粘附性,脱离性,细胞毒性 (乳酸脱酶试验) 和细胞致病性试验.
- 在特定条件下 (30°C24小时) 评估了DES配方对Acanthamoeba castellanii (T4基因型) 的疗效.
主要成果:
- 德斯2表现出最高的氨杀菌活性,在30°C的24小时内达到94%的阿坎萨莫巴清除率.
- 深层欧性溶剂显著降低了对人类大脑内皮细胞的氨基粘附,DES2显示88%的减少.
- 德斯3显示出强大的抗脱落性质,抑制了94%的囊发芽.
- 德斯配方,特别是德斯2,减少了阿坎萨摩巴诱导的内皮细胞死亡,同时表现出最小的细胞毒性,除了母盐.
结论:
- 以化为基础的深溶解溶剂,特别是DES2和DES3,显示出作为抗菌剂对抗致病性菌的显著前景.
- 这些DES配方具有双重活性,抑制双重和囊阶段,并保护宿主细胞.
- 对这些DES的进一步研究可能会导致对阿坎萨摩巴菌感染的新型治疗策略的开发.
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