双循环并列质量用于识别和定量酸丁胆使用异六复合毛细血管nLC-MS/MS
Mahmoud Elhusseiny Mostafa1, Julius Agongo1, Scott F Grady1
1Department of Chemistry and Biochemistry, Saint Louis University, 3501 Laclede Avenue, St. Louis, Missouri 63103, United States.
Journal of the American Society for Mass Spectrometry
|June 13, 2024
概括
研究人员开发了一种新的六个复合体异标记策略,用于分析酸丁胆 (PC) 和酸丁胆 (LPC). 这种方法增强了多重复合能力,改善了生物样本中这些重要的脂质的定量分析.
科学领域:
- 利皮多米克 (Lipidomics) 是一种消化剂.
- 分析化学 分析化学
- 生物化学 生物化学
背景情况:
- 酸丁 (PC) 和酸丁 (LPC) 的分析在生物研究中至关重要.
- 目前用于PC/LPC分析的多重复合方法受到衍生化挑战的限制.
研究的目的:
- 为增强PC和LPC的复杂化和定量化开发一种新的六个复合体同标记策略.
- 克服PC/LPC分析当前衍生技术的局限性.
主要方法:
- 开发了一个新的标记方案,使用针对酸基组的四元氨基标签.
- 在定量产量方面,Dimethoxymethyl morpholinium (DMTMM) 激活确保了>99%的反应完成.
- 选择性同位素结合产生了用于质谱的六个复合组的同位素试剂.
主要成果:
- 标记策略成功地将PC/LPC的净负荷从中性转变为+2.2.
- 碎片化分析产生了一种独特的双循环常数质量报告器.
- 在没有信号压缩的情况下,六倍标签实现了<14%的相对标准偏差 (RSD) 和1:10的动态范围.
- 在人类肝癌细胞中对PCs/LPCs的定量分析成功地使用了六倍数策略进行.
结论:
- 已经建立了一个新的PC/LPC分析的六个plex定性和定量等离子标记策略.
- 这种方法扩大了酸盐胆和酸盐胆量化的分析极限.
- 开发的策略为脂质组学研究提供了更好的多重和准确性.
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