协同转录的拼接促进了巨大的基因的转录
Jaclyn M Fingerhut1,2, Romain Lannes1, Troy W Whitfield1
1Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, United States of America.
PLoS genetics
|June 13, 2024
概括
德洛索菲拉Y链基因中的巨大内子需要共同转录拼接才能成功转录和基因表达. 这一过程防止了转录的纠,确保成熟的信使RNA的产生.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 草虫遗传学 草虫遗传学
背景情况:
- 内子通常很小,但有些基因具有异常大的内子 (数百基基基基基基基基基).
- 这些巨大的内子的功能意义和转录和处理的细胞机制在很大程度上是未知的.
- 德洛索菲拉Y染色体包含一些已知最大的基因,其内子占其跨度的99%以上.
研究的目的:
- 研究共转录拼接在Drosophila中巨大的Y链基因表达中的作用.
- 了解细胞如何管理转录和RNA处理,这是极大的内核所带来的挑战.
主要方法:
- 在Drosophila Y-linked基因中的拼接乱.
- 对转录水平和成熟的信使RNA生产的分析.
- 核转录组织的细胞学检查.
主要成果:
- 破坏巨大的Y链基因的共同转录拼接导致了转录衰减和不能产生成熟的信使RNA.
- 缺陷的拼接导致了无组织的核转录,这表明未拼接的长RNAs纠.
- 共同转录拼接似乎对于保持新生的转录长度低于关键值至关重要.
结论:
- 协同转录拼接对于成功转录和表达Drosophila中巨大的Y链基因至关重要.
- 这一过程防止了转录的纠,从而确保了适当的基因表达.
- 这项研究强调了共转录拼接在管理极大的内核中具有新的生物学意义.
相关概念视频
RNA Splicing
56.3K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
56.3K
Alternative RNA Splicing
21.1K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
21.1K
Organization of Genes
68.5K
Overview
68.5K
Pre-mRNA Processing: RNA Splicing
5.2K
5.2K
What is Gene Expression?
8.5K
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
8.5K
DNA-only Transposons
14.4K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
The donor site from where the transposon is excised is either degraded or...
14.4K


