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上转化纳米粒子基于点点免疫测试用于定量生物标志物检测.

Jakub Máčala1, Ekaterina Makhneva1, Antonín Hlaváček2

  • 1Department of Biochemistry, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.

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光子上转化纳米粒子 (UCNPs) 显著提高了点点免疫测试的灵敏度,用于检测低丰度生物标志物. 这种基于UCNP的测定方法为临床诊断提供了改进的定量分析.

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科学领域:

  • 生物医学工程 生物医学工程
  • 纳米技术 纳米技术
  • 分析化学 分析化学

背景情况:

  • 点点免疫测试对于生物标志物检测是常见的,但对于定量分析往往缺乏灵敏度.
  • 现有的测定通常仅限于定性或半定量测量.
  • 光学背景干扰可能会阻碍传统测试中的敏感检测.

研究的目的:

  • 开发一种使用光子上转换纳米粒子 (UCNPs) 进行定量生物标志物检测的高度敏感的点点免疫试验.
  • 为了克服传统的点点测试的灵敏度限制.
  • 证明基于UCNP的检测对各种临床生物标志物的多功能性和稳定性.

主要方法:

  • 优化使用UCNP作为标签对酸纤维素膜进行点点免疫试验.
  • 对人血清白蛋白 (HSA) 的定量分析,以确定测试性能指标.
  • 将UCNP标签与商业量子点进行灵敏度和信号与背景比率的比较.
  • 测试的验证使用尖的尿液和血清样本检测前列腺特异性抗原 (PSA) 和心脏素 (cTn).
  • 对临床患者样本的分析和与电化学发光免疫检测 (ECLIA) 结果的相关性.

主要成果:

  • 基于UCNP的点点测试实现了HSA的0.19 ng/mL的检测极限 (LOD),信号与背景比 (S/B) 为722.
  • 与量子点相比,UCNPs表现出更高的性能,LOD为4.32 ng/mL,S/B为3.
  • 该试验成功检测了PSA (LOD 9.4 pg/mL) 和cTn (LOD 0.62 ng/mL) 在增值的血清样本中.
  • 对临床样本的分析显示,与ECLIA有很强的相关性,康复率在89%至117%之间.

结论:

  • 光子上转换纳米粒子作为点点免疫测试的高度敏感标签.
  • 基于UCNP的点点测试可以定量检测低丰度生物标志物.
  • 这项技术为敏感和强大的临床诊断提供了一个有前途的平台.