相关实验视频

Updated: May 6, 2026

Early Viral Entry Assays for the Identification and Evaluation of Antiviral Compounds

Early Viral Entry Assays for the Identification and Evaluation of Antiviral Compounds

Published on: October 29, 2015

绿色和多功能高通量微波氧化基光谱光度法用于囊中的加利迪西维尔的确定

Ibrahim A Darwish¹, Nourah Z Alzoman¹

¹Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, PO Box 2457, Riyadh 11451, Saudi Arabia.

Journal of AOAC International

|June 13, 2024

概括

新的光谱光度法确定了散装和囊中的加利迪西维尔 (GDV). 这些多功能,绿色微波分析为制药制造提供了准确的量化.

科学领域:

分析化学分析化学
药品分析药品分析

背景情况:

加利迪西维尔 (Galidesivir,简称GDV) 是一种强效的抗病毒药物,具有广泛的疗效,包括对抗COVID-19.
没有确定的分析方法可以量化大量或剂量形式的GDV.

研究的目的:

开发多功能,绿色和简单的微波光谱法 (MW-SPMs) 用于GDV的确定.
在散装材料和囊配方中量化GDV.

主要方法:

三个MW-SPM使用甲酸盐,三氧化物和酸酸盐进行开发.
在室温的96井板中进行了反应,并使用微板阅读器测量了吸收率.
关键反应变量被优化以提高性能.

主要成果:

对于GDV度在25-500μg/mL之间观察到非常好的线性 (R2=0.9991-0.9997).
获得了高精度 (98.6-101.2%的恢复) 和精度 (RSD ≤1.02%).
这些方法在散装和囊中成功应用于GDV分析,证实了它们的绿色凭证.

结论:

开发的MW-SPM提供了高吞吐量,自动化准备和精确的量化.
使用常见的,具有成本效益的实验室试剂可以提高方法的实用性和安全性.
这些MW-SPM是整个制药制造过程中GDV量化的有价值工具.

更多相关视频

A High-throughput Compatible Assay to Evaluate Drug Efficacy against Macrophage Passaged Mycobacterium tuberculosis

A High-throughput Compatible Assay to Evaluate Drug Efficacy against Macrophage Passaged Mycobacterium tuberculosis

Published on: March 24, 2017

A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials

A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials

Published on: March 3, 2023

相关概念视频

In Vitro Drug Dissolution: Alternative Methods

In Vitro Drug Dissolution: Alternative Methods

Alternative drug dissolution methods include the rotating bottle, intrinsic dissolution test, peristalsis, and the Franz diffusion cell method. The rotating bottle method involves meticulously rotating tightly capped controlled-release beads in a temperature-controlled bath. Periodic decanting of samples allows for residue assay, followed by refilling with fresh medium and testing at various pH levels to emulate the gastrointestinal tract conditions.In contrast, the intrinsic dissolution test...