相关实验视频

Updated: Jun 24, 2025

Reinstatement of Drug-seeking in Mice Using the Conditioned Place Preference Paradigm

Reinstatement of Drug-seeking in Mice Using the Conditioned Place Preference Paradigm

Published on: June 7, 2018

通过KDM1A对表观遗传调节的PP1α表达可能有助于在小鼠中氧化条件下的位置偏好

Hong-Xi Li¹, Li-Yu Yang², Yu-Xiao Wan³

¹Department of Pain Management, Shengjing Hospital of China Medical University, Shenyang, China.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

|June 13, 2024

概括

氨酸特异性去甲基酶1 (KDM1A) 影响了氧化奖励记忆. 抑制KDM1A可以阻止氧化.

科学领域:

神经科学是一个神经科学.
分子生物学分子生物学
药理学药理学是指药理学的学科.

背景情况:

氨酸特异性去甲基酶1 (KDM1A) 是已知的学习和记忆的调节者.
对于KDM1A在氧化的奖励作用中的作用仍然未被探索.

研究的目的:

研究KDM1A在氧化相关奖励记忆的形成和表达中的作用.
阐明KDM1A参与氧化奖励的基础分子机制.

主要方法:

在雄性小鼠中的条件位置偏好 (CPP) 范式来评估奖励记忆.
下一代测序和染色体免疫沉-PCR用于分析分子变化.
药物抑制 (ORY-1001) 和基因淘汰 (siRNA/shRNA) 的KDM1A.

主要成果:

氧化的使用降低了海马神经元中的PP1αmRNA和蛋白质水平.
氧化增加了KDM1A和H3K4me1,同时降低了H3K4me2水平.
KDM1A的抑制或淘汰阻止了氧化CPP的获取/表达,并促进了灭绝.
抑制KDM1A阻止了氧化诱导的PP1α和CoRest与PP1α促进体结合的减少.

结论:

氧化可调节KDM1A,可能通过H3K4me2脱甲基化.
这种KDM1A上调促进了CoRest与PP1α促进体的结合,减少了PP1α的表达.
在海马神经元中,KDM1A介导的PP1α表达的降低有助于氧化奖励.

关键词:

在 CoRest 休息.有条件的地方偏好.表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.在KDM1A中,KDM1A是KDM1A.氧化可 (Oxycodone) 是一种氧化.

更多相关视频

A Conditioned Place Preference Protocol for Measuring Incubation of Craving in Rats

A Conditioned Place Preference Protocol for Measuring Incubation of Craving in Rats

Published on: November 6, 2018

Assessment of Cocaine-induced Behavioral Sensitization and Conditioned Place Preference in Mice

Assessment of Cocaine-induced Behavioral Sensitization and Conditioned Place Preference in Mice

Published on: February 18, 2016

相关概念视频

Analgesia and Pain Management

Analgesia and Pain Management

Pain is critical to various clinical pathologies, provoking an urgent need for effective management. Pain, whether acute or chronic, is a complex neurochemical process. Its alleviation depends on the type, with nonopioid analgesics effective for mild to moderate pain, such as musculoskeletal or inflammatory pain, while neuropathic pain responds best to anticonvulsants, tricyclic antidepressants, or serotonin/norepinephrine reuptake inhibitors. For severe acute or chronic pain, opioids may be...

Opioid Receptors: Overview

Opioid Receptors: Overview

Opioid receptors, including the mu (μ, MOR), delta (δ, DOR), and kappa (κ, KOR) types, belong to the rhodopsin family of G protein-coupled receptors. These receptors are located throughout the central and peripheral nervous systems and in non-neuronal tissues such as macrophages and astrocytes. Opioid receptor ligands can be categorized into agonists or antagonists. Highly selective agonists include [d-Ala2, MePhe4, Gly(ol)5]-enkephalin or DAMGO for MOR, [D-Pen2,...

Epigenetic Regulation

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...

Genomic Imprinting and Inheritance

Genomic Imprinting and Inheritance

Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...