冷却和冷保护剂对胚胎中的甘氨酸的影响
Kanokpron Loeslakwiboon1, Hsing-Hui Li2, Sujune Tsai3
1Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung, Taiwan; National Museum of Marine Biology & Aquarium, Pingtung, Taiwan; Graduate Institute of Marine Biology, National Dong Hwa University, Pingtung, Taiwan.
Cryobiology
|June 13, 2024
概括
这项研究揭示了冷却和冷保护剂如何影响胚胎中的甘氨酸. 曼诺斯是胚胎生长的关键,而冷却会改变其他甘氨酸,帮助寒冷适应.
科学领域:
- 海洋生物学 海洋生物学
- 发育生物学是发展生物学.
- 生物化学 生物化学
背景情况:
- 甘氨酸是一种必需的碳水化合物,参与细胞增殖和蛋白质贩运.
- 胚胎发生是一个关键的发育阶段,特别是在海洋生物中.
- 了解压力下的甘氨酸变化对于海洋物种的保护至关重要.
研究的目的:
- 为了研究冷却和冷保护剂 (CPAs) 对Stenopus hispidus胚胎中的甘氨基基形状的影响.
- 确定参与胚胎发育和寒冷应激反应的关键甘氨酸.
- 评估CPA在低温暴露期间对胚胎的保护作用.
主要方法:
- 在S.hispidus胚胎中使用莱克阵列在心跳阶段分析糖甘配置.
- 用各种冷保护剂 (CPA) 处理胚胎.
- 在冷却和CPA处理之前和之后对糖甘含量的比较分析.
主要成果:
- 曼诺被确定为最丰富的甘氨酸,对胚胎细胞增殖和能源供应至关重要.
- 冷却显著改变了诸如糖和N-乙葡萄糖胺 (GlcNAc) 等甘氨酸的含量,这可能有助于通过单糖累积来适应寒冷.
- 在CPA治疗后,某些特定的单蛋白 (例如NPA, discoidin I) 的结合发生了变化,而DMSO可能会减轻冷却引起的细胞损伤.
结论:
- 这项研究提供了在低温压力下胚胎中的甘氨酸样本的首次全面分析.
- 这些发现增强了对甘氨酸在胚胎发生和细胞适应环境挑战中的作用的理解.
- 这项研究为冷保存策略和了解海洋无脊椎动物的发育生物学提供了宝贵的见解.
相关概念视频
02:45Responses to Heat and Cold Stress
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
03:12Freezing Point Depression and Boiling Point Elevation
Boiling Point Elevation
The boiling point of a liquid is the temperature at which its vapor pressure is equal to ambient atmospheric pressure. Since the vapor pressure of a solution is lowered due to the presence of nonvolatile solutes, it stands to reason that the solution’s boiling point will subsequently be increased. Vapor pressure increases with temperature, and so a solution will require a higher temperature than will pure solvent to achieve any given vapor pressure, including one...
The boiling point of a liquid is the temperature at which its vapor pressure is equal to ambient atmospheric pressure. Since the vapor pressure of a solution is lowered due to the presence of nonvolatile solutes, it stands to reason that the solution’s boiling point will subsequently be increased. Vapor pressure increases with temperature, and so a solution will require a higher temperature than will pure solvent to achieve any given vapor pressure, including one...
01:15Transcytosis of IgG
Transcytosis is the process in which molecules are internalized by endocytosis, transported across the cell, and released through exocytosis from the opposite end of the cell. Molecules such as insulin, immunoglobulins, and certain nutrients are transferred through the recycling endosomes by recycling and transcytosis.
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
01:07Teratogenicity
The ability of a drug to produce structural deformations and functional abnormalities in the developing embryo or the fetus is called teratogenicity, and the drug producing this effect is known as a teratogen. Teratogenic effects include stillbirth, miscarriage, intrauterine growth restriction, and neurocognitive delay. A teratogen may affect the embryo at different stages of development, which is important in determining the type and extent of the damage. During blastocyst formation, the early...
01:19Retarders
Retarders are chemical admixtures designed to extend the setting time, which is especially useful when there is a delay in sequential concrete pours to prevent cold joints and to achieve a cohesive structure. Retarders, when used in appropriate amounts, can also enhance the architectural appearance of exposed aggregate finishes.
The function of retarders is to delay the setting of concrete, and this effect can be measured using a penetration test. The retardation process involves adding...
The function of retarders is to delay the setting of concrete, and this effect can be measured using a penetration test. The retardation process involves adding...