miR-504淘汰赛调节瘤细胞的增殖和免疫细胞的透,以加速口腔癌的发展
Xiaotang Wang1, Xiaona Song1, Yunhui Ma1
1Laboratory Animal Center, Shanxi Medical University, Taiyuan, Shanxi 030001, China; Department of Basic Medical Sciences, Shanxi Medical University, Taiyuan, Shanxi 030001, China.
Journal of genetics and genomics = Yi chuan xue bao
|June 13, 2024
概括
在小鼠中,微RNA-504 (miR-504) 损失加速了口腔癌的进展. 这通过改变脂代谢和免疫细胞透到瘤微环境中来发生,突出显示miR-504
科学领域:
- 分子生物学分子生物学
- 在瘤学瘤学.
- 遗传学 是一个遗传学.
背景情况:
- 微RNA-504 (miR-504) 涉及口腔癌的进展,但其体内机制尚未完全理解.
- 在口腔癌患者中,miR-504显著下调,这表明它具有抑制瘤的作用.
研究的目的:
- 调查miR-504在口腔癌发展中的体内作用.
- 阐明miR-504影响口腔癌进展的分子机制.
主要方法:
- 使用CRISPR/Cas9技术生成了miR-504淘汰赛小鼠 (miR-504-/-) .
- 在使用4-Nitroquinoline N-oxide (4NQO) 暴露的小鼠中诱导了口腔癌.
- 分析病理变化,血液生化指数,并进行RNA测序.
主要成果:
- miR-504淘汰并没有影响小鼠的一般特征,但改变了血液样本.
- miR-504-/-小鼠在4NQO治疗后表现出恶化的口腔癌病理.
- RNA测序揭示了代谢,细胞因子和脂质通路中的基因表达改变,特别是影响免疫细胞透.
结论:
- 在4NQO诱导的小鼠模型中,miR-504的损失加速了口腔癌的进展.
- 这种加速与失调的瘤细胞增殖,改变的脂质代谢和瘤微环境中的修饰免疫细胞透有关.
相关概念视频
02:43Mouse Models of Cancer Study
5.5K
Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
5.5K
01:22MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K