体表达识别了古典NK细胞的早期骨髓前体
Zhitao Liang1,2, Hope D Anderson3, Veronica Locher1
1Committee on Immunology, University of Chicago, Chicago, IL, USA.
Nature immunology
|June 13, 2024
概括
研究人员确定了EomeshiNKneg细胞作为自然杀手 (NK) 细胞的骨髓前体. 这些发现表明,NK细胞和1型先天性淋巴细胞 (ILC1s) 在发育早期分离.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 发展生物学 发展生物学
背景情况:
- 自然杀手 (NK) 细胞是细胞毒性淋巴细胞,对免疫对病原体和瘤至关重要.
- 1型先天性淋巴细胞 (ILC1s) 与NK细胞共享细胞因子的生产,但存在于组织中,缺乏细胞毒性.
- NK细胞和ILC1s之间的发育关系尚不清楚,其中可能包括不同的血统或共享的前体.
研究的目的:
- 研究NK细胞和ILC1s的发育起源.
- 识别产生NK细胞和ILC1s的前体种群.
- 为了确定NK细胞和ILC1s是否来自共同的祖先或单独的血统.
主要方法:
- 单细胞RNA测序被用来分析细胞群.
- 流细胞测量用于表征细胞表面标记物和转录因子表达.
- 在体内进行细胞转移实验时,使用Rag2 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 的受体小鼠.
主要成果:
- 鉴定出了一种独特的骨髓谱系阴性,NK受体阴性细胞,表达高水平的转录因子Eomes (EomeshiNKneg).
- 将EomeshiNKneg细胞转移到免疫缺陷受体中,产生了表现出抗转移活性的功能NK细胞.
- 转移PLZF+的ILC前体产生了缺乏细胞毒性功能的ILC子集 (ILC1s,ILC2s,ILC3s) 的混合物.
结论:
- 体NK细胞代表了古典NK细胞的特定骨髓前体.
- 这项研究提供了证据,表明NK细胞和ILC1系在发育早期有分歧.
- 这项研究澄清了先天性淋巴细胞和NK细胞的发育轨迹.
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