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立体辐射疗法通过调节miR-21/Sorbin和SH3域含蛋白1轴来抑制骨髓衍生抑制细胞

ChunFang Zhao¹, Qi Tang², Congbo Yang¹

¹Department of Radiation Oncology, The Third Affiliated Hospital of Kunming Medical University, Kunmming, China.

Human & experimental toxicology

|June 14, 2024

概括

在肺癌模型中,立体体辐射疗法 (SBRT) 增加了miR-21,抑制了髓质衍生抑制细胞 (MDSC) 扩张. 这表明miR-21上调是SBRT的关键.

科学领域:

在瘤学瘤学.
免疫学免疫学免疫学
分子生物学分子生物学

背景情况:

立体性身体辐射疗法 (SBRT) 是用于早期癌症的局部辐射技术.
对于SBRT对髓衍生抑制细胞 (MDSC) 的作用尚不清楚.
调查SBRT对MDSC分化和生存的影响对于了解治疗疗效至关重要.

研究的目的:

检查SBRT如何影响MDSC分化和生存.
阐明SBRT对MDSC的影响背后的分子机制.
在肺癌模型中评估miR-21在SBRT介导的免疫调节中的作用.

主要方法:

流细胞计分析了骨髓 (BM) 细胞中的MDSC分化和亡.
一个肺癌小鼠模型被用来评估SBRT的抗癌作用.
通过RT-qPCR,Western blot和双露西法酶测定,探索了miR-21/SORBS1轴.

主要成果:

SBRT增加了miR-21水平,抑制了MDSC分化,并在BM细胞中诱导了亡.
抑制miR-21逆转了SBRT对MDSC分化和亡的影响.
确定了miR-21/SORBS1通路作为SBRT诱导的MDSC变化的关键调节器.
在体内miR-21调制影响了肺癌模型中的SBRT反应和MDSC水平.

结论:

由SBRT诱导的miR-21上调抑制了肺癌中MDSC扩张.
miR-21/SORBS1轴是SBRT免疫调节效应的关键调解器.
向miR-21可能会提高肺癌治疗中的SBRT疗效.

关键词:

在SORBS1中,我们可以选择SORBS1.刻板印象性的身体辐射疗法肺癌是一种肺癌.据报道,米R-21的发射器为米R-21 来自骨髓细胞的抑制细胞.

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