本质上有障碍的区域调节细菌中的RhlE RNA螺旋酶功能
Stéphane Hausmann1, Johan Geiser1, George Edward Allen1
1Department of Microbiology and Molecular Medicine, CMU, Faculty of Medicine, University of Geneva, Geneva, Switzerland.
Nucleic acids research
|June 14, 2024
概括
在Pseudomonas aeruginosaRNA螺旋酶RhlE1和RhlE2中的内在无序区域 (IDR) 通过调节RNA结合,相分离和与RNase E.等其他细胞组件的相互作用来决定它们的独特功能.
科学领域:
- 分子生物学分子生物学
- 生物化学 生物化学
- 微生物学 微生物学
背景情况:
- RNA螺旋酶对RNA代谢至关重要,通常具有内在无序区域 (IDR),促进复杂的相互作用和相分离.
- Pseudomonas aeruginosa 使用两个非冗余RNA螺旋酶,RhlE1和RhlE2,它们共享一个催化核,但在C端IDR上有所不同.
研究的目的:
- 研究RhlE1和RhlE2RNA螺旋体之间的内在无序区域 (IDR) 的变异如何赋予功能特异性.
- 阐明IDRs在RNA结合,相分离,酶活性和与其他细胞因子相互作用中的作用.
主要方法:
- 用交换的IDR构建和分析嵌合式RNA基酶.
- 生物化学测试以评估RNA解活动.
- 对蛋白质与蛋白质相互作用的分析,特别是对RNase E.的分析.
- 在不同温度条件下的细菌生长的评估.
- 在细胞质内对RNA酶的局部化研究.
主要成果:
- 无论是RhlE1和RhlE2的IDR都促进RNA结合和细胞质相分离.
- 与RhlE1 IDR相比,RhlE2 IDR更有效地增强了RNA解活动和相位分离.
- 该RhlE2 IDR调解与RNase E内核酶的相互作用.
- 与原生形式相比,化学环酶表现出不同的生化和功能性质.
- 这种RECRhlE1-IDRRhlE2仿真体改善了寒冷生长,并与RNase E.相互作用.
- 在低温下,RECRhlE2-IDRRhlE1仿真体损害了细菌的生长,这与异常的RNA滴有关.
结论:
- 内在无序区域 (IDR) 是RNA基酶功能特异性的关键决定因素.
- IDRs通过相分离调节催化核心活动和RNA酶的亚细胞局部化.
- 了解IDR多样性对于破译像Pseudomonas aeruginosa这样的细菌中RNA基酶的功能差异化至关重要.
相关概念视频
01:23Types of RNA
63.5K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
63.5K
00:43Bacterial RNA Polymerase
29.4K
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
29.4K
00:55DNA Helicases
21.3K
DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
21.3K
02:47Ribozymes
11.2K
The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can...
Ribozymes can...
11.2K
03:01The Replisome
33.4K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
33.4K
02:42Transcription Attenuation in Prokaryotes
15.3K
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
15.3K