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转录后修改以调节异型球体上的原始基因分化

Nazmiye Celik^1,2, Srinivas V Koduru³, Dino J Ravnic⁴

¹Engineering Science and Mechanics Department, The Pennsylvania State University, University Park, Pennsylvania, USA.

Tissue engineering. Part A

|June 14, 2024

概括

微RNAs (miRs) 指导干细胞分化,用于创建血管化骨组织模型. 优化诱导时间是成功培和潜在的3D生物打印应用的关键.

科学领域:

生物材料科学生物材料科学
干细胞生物学干细胞生物学
组织工程是组织工程.

背景情况:

细胞聚合物对于在体外研究血管化至关重要.
了解异型细胞相互作用对于组织发育至关重要.

研究的目的:

调查微RNAs (miRs) 在诱导细胞球体中的骨质和内皮质分化中的作用.
评估miR治疗和诱导时间对血管化骨建模的编程差异化的影响.

主要方法:

使用脂肪衍生干细胞和内皮前代细胞构建的异型球形.
感染了miR-148b模仿骨质生成的细胞和miR-210模仿内皮形成的细胞.
培养球体4周,分析基因/蛋白质标记物和矿物化.

主要成果:

微RNAs有效地引导细胞分化向骨质原生和内皮原生血统.
原生细胞成熟度显著影响了聚合物内的异型细胞区域的形成.
聚合前的诱导时间是成功进行代分化的关键因素.

结论:

这项研究展示了一种新的方法,使用miRs指导分化,用于创建预血管化骨组织模型.
优化分化诱导的时间对于成功的培养是必不可少的.
这种基于球体的方法有可能用于先进的组织工程应用,包括3D生物打印.

关键词:

骨头骨头骨头骨头内皮质生成 (endotheliogenesis) 是一种内皮质形成的过程.这是一个小RNARNA.骨质发生过程 (osteogenesis)偏神经信号传递系统原始人的祖先.球形状的球形体是指球形状的球体.血管化的血管化.

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