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Updated: Jun 23, 2025

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
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定向进化突变增强了腺基编辑器ABE8e的DNA结合亲和力和蛋白质稳定性

Haixia Zhu1, Lei Wang1, Ying Wang1

  • 1State Key Laboratory of Genetic Engineering, Shanghai Engineering Research Center of Industrial Microorganisms, MOE Engineering Research Center of Gene Technology, School of Life Sciences, Fudan University, Shanghai, 200438, China.

Cellular and molecular life sciences : CMLS
|June 14, 2024
PubMed
概括

定向进化突变通过增加DNA结合和蛋白质稳定性来增强腺基编辑器 (ABEs). 在TadA8e中的关键突变改善了ABE8e.

关键词:
在ABE8e中,ABE8e是ABE8e.基础编辑器 基础编辑器结合 DNA 的 DNA 结合.静电相互作用 静电相互作用基因组编辑 基因组编辑热稳定性 热稳定性

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