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甲醇作为与CO2的共同基质增强了微生物电合成中的丁酸盐生产

Hui Yao¹, Johanna M Rinta-Kanto¹, Igor Vassilev¹

¹Faculty of Engineering and Natural Sciences, Tampere University, Korkeakoulunkatu 8, 33720, Tampere, Finland.

Applied microbiology and biotechnology

|June 14, 2024

概括

醇和二氧化碳 (CO2) 进入微生物电合成 (MES) 显著增加了酸盐的生产. 甲醇作为电子和碳来源,提高了产量,而不是仅仅是CO2.

科学领域:

生物技术和生物工程生物技术和生物工程
可持续化学可持续化学
微生物电合成微生物电合成

背景情况:

甲醇是生物基础经济的多功能C1原料,可以从废物或CO2电化学中获得.
微生物电合成 (MES) 正在探索从二氧化碳中生产有价值的化学物质,但酸盐比乙酸盐更受关注.
优化MES的价值更高的产品,如丁酸盐需要探索替代或共同基质.

研究的目的:

通过使用甲醇作为与CO2的共同基质来研究在MES中增强丁酸盐生产.
阐明甲醇作为电子捐赠体和碳源在微生物酸盐电合成中的作用.
为了确定微生物群落的转移和在MES中酸盐生产期间丰富的占主导地位的属.

主要方法:

使用微生物电合成 (MES) 反应器,用二氧化碳和甲醇混合物养.
在不同的料条件下量化酸盐生产率和度 (仅CO2与CO2+甲醇).
分析甲醇对生产的酸盐碳池的贡献.
使用16S rRNA基因测序来表征微生物社区的组成.

主要成果:

与二氧化碳和甲醇同时养的结果是,与单独的二氧化碳 (0.20 g L-1 d-1和5.2 g L-1) 相比,酸盐的生产率 (0.36 g L-1 d-1) 和度 (8.6 g L-1) 显著更高.
甲醇充当了电子捐赠者和碳来源,对产生的丁酸盐的碳含量贡献了约50%.
在优化条件下,微生物群体主要由Eubacterium属 (52.6%的相对丰度) 主导.

结论:

甲醇与二氧化碳联合养是一种有效的策略,用于增强微生物电合成中的丁酸盐生产.
甲醇作为电子和碳来源的双重作用对于提高产品产量至关重要.
丰富特定的微生物属,如Eubacterium,是MES中C1基质有效转化为丁酸盐的关键.

关键词:

黄糖酸盐是什么黄糖酸盐使用的二氧化碳利用率.电子捐赠者是电子的捐赠者甲醇利用率的使用方法微生物的电合成.

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