相关实验视频

Updated: Jun 23, 2025

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells

Published on: May 4, 2018

维生素D补充剂会影响骨髓衍生中酶体干细胞分化成产生胰岛素的细胞

Marzieh Nemati¹, Ali Akbar Alizadeh^1,2, Sanaz Dastghaib^1,3

¹Endocrinology and Metabolism Research Center, Shiraz University of Medical Science, Shiraz, Iran.

Molecular biology reports

|June 14, 2024

概括

维生素D增强了骨髓衍生中酶干细胞 (BM-MSCs) 转化为胰岛素生成细胞 (IPCs) 的分化. 这一发现表明维生素D作为一种潜在的补充剂,可以改善干细胞分化成产生胰岛素的细胞.

科学领域:

干细胞生物学干细胞生物学
内分泌学在内分泌学.
生物化学生物化学

背景情况:

介质干细胞 (MSC) 是多能细胞,具有分化成各种细胞类型的潜力.
胰岛素生成细胞 (IPC) 对葡萄糖平衡至关重要,它们的功能障碍与糖尿病有关.
维生素D是一种类固醇激素,已知在平衡和免疫功能中的作用,以及在细胞分化中的新兴作用.

研究的目的:

为了研究维生素D对大鼠骨髓衍生中酶干细胞 (BM-MSCs) 转化为IPCs的差异化的作用.
评估维生素D如何影响与IPC分化和胰岛素分泌相关的基因表达.
评估维生素D对分化细胞活力和胰岛素含量的影响.

主要方法:

从大鼠的股骨和骨中分离出了BM-MSCs.
细胞在低葡萄糖 (LG),高葡萄糖 (HG) 或高葡萄糖与维生素D (HGD) 介质中培养了14天.
通过MTT测定来评估细胞活力;通过测量Pdx1和胰岛素基因表达,通过ELISA测量胰岛素分泌和含量来确认分化.

主要成果:

与LG和HG组相比,HGD中的细胞活力在第3天和第7天显著更高.
与LG相比,HGD组的Pdx1和胰岛素基因表达水平明显高于HGD组.
与对照组相比,在低血糖和高血糖条件下,在用维生素D治疗的细胞 (HGD) 中,胰岛素分泌和含量显著增加.

结论:

维生素D补充剂显著提高BM-MSCs的分化到IPCs.
维生素D有助于增加Pdx1和胰岛素表达,以及改善胰岛素分泌和含量.
维生素D显示出作为补充剂的潜力,可以改善干细胞分化成产生胰岛素的细胞.

关键词:

骨髓衍生中酶体干细胞的骨髓衍生干细胞.糖尿病糖尿病糖尿病不同化的差异化产生胰岛素的细胞产生胰岛素.治疗方法治疗方法

更多相关视频

Isolation of Rat Adipose Tissue Mesenchymal Stem Cells for Differentiation into Insulin-producing Cells

Isolation of Rat Adipose Tissue Mesenchymal Stem Cells for Differentiation into Insulin-producing Cells

Published on: August 29, 2022

Transient Treatment of Human Pluripotent Stem Cells with DMSO to Promote Differentiation

Transient Treatment of Human Pluripotent Stem Cells with DMSO to Promote Differentiation

Published on: July 17, 2019

相关概念视频

Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...

iPS Cell Differentiation

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.

Role of Skin in Vitamin D Synthesis

Role of Skin in Vitamin D Synthesis

The skin plays a crucial role in the synthesis of vitamin D, a vital nutrient for various physiological processes in the body. Vitamin D is unique because it can be synthesized in the skin through a series of chemical reactions triggered by exposure to ultraviolet B (UVB) radiation from sunlight.
The solar UV B rays (290-315 nm) are absorbed by the skin, and 7-dehydrocholesterol (provitamin D3) photolyzes it to previtamin D3, which undergoes a rapid transformation to vitamin...