长相核心蛋白和A类PBP1a在Streptococcus pneumoniae中细胞中显示区域性,过程性运动
Amilcar J Perez1, Melissa M Lamanna1, Kevin E Bruce1
1Department of Biology, Indiana University Bloomington, Bloomington, IN 47405.
概括
细菌细胞壁的合成涉及两个关键的蛋白质复合体. 肺炎球菌延长体蛋白,bPBP2b,Roda和MreC,在细胞中间环形移动,由细胞壁合成驱动.
科学领域:
- 微生物学 微生物学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 肺炎 estreptococcus 使用两个空间分离的甘氨酸 (PG) 合成酶复合体进行细胞分裂.
- 隔膜PG合成酶关闭细胞隔膜,而延长体合成了外围PG.
- 以前的研究表明,隔膜PG合成酶的运动是由PG合成驱动的.
研究的目的:
- 为了研究肺炎球菌延长体蛋白的动态.
- 确定PG合成在长相蛋白运动中的作用.
- 了解长相组件的相互作用和定位.
主要方法:
- 使用单分子全内反射光显微镜 (sm-TIRFm) 来追踪蛋白质动态.
- 有关蛋白质包括bPBP2b,Roda,MreC,aPBP1a和MpgA.
- 用基因枯竭来评估蛋白质依赖性.
主要成果:
- 长相蛋白质bPBP2b,Roda和MreC在PG合成的驱动下,在细胞中间表现出环形运动.
- 在中细胞外,这些蛋白质表现出扩散运动.
- bPBP2b和Roda在定位和运动方面相互依赖;MreC的耗尽会破坏bPBP2b的运动.
- aPBP1a的一小部分也在周围运动,而MpgA在中细胞表现出有限的亚扩散运动.
结论:
- 肺炎球菌延长体蛋白表现出不同的动态行为,这取决于PG合成和蛋白质相互作用.
- 核心延长体组件的周围运动对于细胞壁在细胞中部的合成至关重要.
- 辅助蛋白如aPBP1a和MpgA具有专门的作用和动态模式.
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