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在拉斯莫分支中开发一个Thromboelastography试验.

Kayla L Bonadie1, Alex M Lynch2, Laura K Ruterbories1

  • 1North Carolina State University College of Veterinary Medicine, Raleigh, NC 27606 USA.

Journal of zoo and wildlife medicine : official publication of the American Association of Zoo Veterinarians
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概括

使用新鲜全血和RapidTEG,为elasmobranchs建立一个可靠的血栓结晶学 (TEG) 协议,对于诊断鱼和鱼的静血障碍至关重要. 这一进步有助于为这些独特的海洋动物提供兽医护理.

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科学领域:

  • 兽医医学 兽医医学 兽医医学
  • 比较生理学比较生理学
  • 海洋生物学 海洋生物学

背景情况:

  • 血栓形成显微镜 (TEG) 是一种有价值的静血检测方法,但由于生理差异和试剂不可用,其在非哺乳动物物种,特别是弹性支柱动物中的应用是有限的.
  • 在elasmobranchs中凝血病的死前诊断是具有挑战性的,阻碍了对静血性或出血性疾病的及时和有效治疗.
  • 现有的静血分析缺乏分类特定的试剂,并与elasmobranchs独特的生理学作斗争.

研究的目的:

  • 为了建立一个标准化的血小板结晶学 (TEG) 协议,用于elasmobranchs.
  • 为了改进血液静止的死前评估在elasmobranchs.
  • 为了促进人类护理下的弹性支架的先进诊断和治疗选择.

主要方法:

  • 评估了多种凝血启动剂 (RapidTEG,引用高,Reptilase,物种大脑衍生型血栓塑) 用不同的弹性枝状血和全血样本.
  • 使用冷解的酸血,新鲜的酸血和新鲜的全酸血来比较TEG结果.
  • 评估了TEG反应在各种试剂和样本类型中的一致性和可靠性.

主要成果:

  • 来自elasmobranchs的血样本显示出不一致的凝血,导致不可靠的TEG结果.
  • 使用新鲜全血的TEG分析始终产生可测量的反应与多个凝血发起剂.
  • 最可靠和可重复的弹性枝状TEG结果是使用新鲜的全血与RapidTEG凝块启动试剂相结合而获得的.

结论:

  • 已经成功地建立了一种使用新鲜全血和RapidTEG的新型弹性枝状血栓结晶造影 (TEG) 协议.
  • 该协议提供了一种可靠的方法来评估弹性枝状血液静止,克服了以前基于等离子体的测试的局限性.
  • 预计开发的TEG方法将在兽医环境中显著提高弹性枝状动物血静性疾病的诊断和管理.